IR@PKUHSC  > 北京大学药学院
学科主题药学
Comprehensive studies on the interactions between chitosan nanoparticles and some live cells
Zheng, Ai-ping2,3; Liu, Hui-xue2; Yuan, Lan2; Meng, Meng2; Wang, Jian-cheng2; Zhang, Xuan2; Zhang, Qiang1,2
关键词Nanoparticles Polycationic Polymers Thymopentin Polymer Interactions Caco-2 Cells C6 Cells Nanomedicine
刊名JOURNAL OF NANOPARTICLE RESEARCH
2011-10-01
DOI10.1007/s11051-011-0447-3
13期:10页:4765-4776
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
资助者National Basic Research Program of China ; State Key Projects ; National Basic Research Program of China ; State Key Projects
研究领域[WOS]Chemistry ; Science & Technology - Other Topics ; Materials Science
关键词[WOS]IN-VITRO CYTOTOXICITY ; RAMAN-SPECTROSCOPY ; DRUG-DELIVERY ; ORAL DELIVERY ; PERMEABILITY ; MOLECULES ; CARRIERS ; FORMULATIONS ; FLUORESCENCE ; SYSTEM
英文摘要

As more and more oral formulations of nanoparticles are used in clinical contexts, a comprehensive study on the mechanisms of interaction between polymer nanoparticles and live cells seems merited. Such a study was conducted and the results were compared to the polymer itself in order to demonstrate different kinds of effects that are brought into the cell by polymer and its nanoparticles, especially the effects on the biomembrane. Several techniques, including surface plasmon resonance (SPR), Fourier transformed infrared spectroscopy (FTIR), Raman spectroscopy, fluorescence polarization spectroscopy (FP), flow cytometry (FCM) with quantitative analysis, and confocal images with antibody staining were employed toward this end. The cytotoxicity in vitro was also evaluated. Chitosan (CS), a polycationic polymer, was used to prepare the nanoparticles. We demonstrate that chitosan nanoparticles (CS-NP) induce strong alterations in the distribution of membrane proteins, fluidity of membrane lipids, and general membrane structure. Furthermore, the uptake of CS-NP into Caco-2 cells was found to have a similar mechanism to that of CS molecules, but the differences in degree were noted. These results indicate that positive charge and nanoscale size were the factors that most significantly affected the interactions between the nanoparticles of polycationic polymers and live cells. However, no difference in cytotoxicity toward the Caco-2 cells was found between CS and CS-NP. This supports the idea that CS-NP is an effective and safe carrier for oral drug delivery.

语种英语
所属项目编号2009CB930300 ; 2009ZX09310-001
资助者National Basic Research Program of China ; State Key Projects ; National Basic Research Program of China ; State Key Projects
WOS记录号WOS:000295609700035
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57743
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
推荐引用方式
GB/T 7714
Zheng, Ai-ping,Liu, Hui-xue,Yuan, Lan,et al. Comprehensive studies on the interactions between chitosan nanoparticles and some live cells[J]. JOURNAL OF NANOPARTICLE RESEARCH,2011,13(10):4765-4776.
APA Zheng, Ai-ping.,Liu, Hui-xue.,Yuan, Lan.,Meng, Meng.,Wang, Jian-cheng.,...&Zhang, Qiang.(2011).Comprehensive studies on the interactions between chitosan nanoparticles and some live cells.JOURNAL OF NANOPARTICLE RESEARCH,13(10),4765-4776.
MLA Zheng, Ai-ping,et al."Comprehensive studies on the interactions between chitosan nanoparticles and some live cells".JOURNAL OF NANOPARTICLE RESEARCH 13.10(2011):4765-4776.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zheng, Ai-ping]的文章
[Liu, Hui-xue]的文章
[Yuan, Lan]的文章
百度学术
百度学术中相似的文章
[Zheng, Ai-ping]的文章
[Liu, Hui-xue]的文章
[Yuan, Lan]的文章
必应学术
必应学术中相似的文章
[Zheng, Ai-ping]的文章
[Liu, Hui-xue]的文章
[Yuan, Lan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。