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学科主题: 临床医学
题名:
Improved RP-HPLC method to determine biapenem in human plasma/urine and its application to a pharmacokinetic study
作者: Zhao, Liho; Liu, Yi; Kou, Zhibin; Bayasi, Aidijie; Cai, Huan; Zhang, Chunyan; Wang, Qian; Li, Yuzhen; Fang, Yi
关键词: Antibacterial ; Biapenem ; CAS 120410-24-4 ; Human plasma and urine ; Pharmacokinetics ; RP-HPLC
刊名: ARZNEIMITTELFORSCHUNG-DRUG RESEARCH
发表日期: 2011
卷: 61, 期:3, 页:197-204
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; MONTE-CARLO-SIMULATION ; IN-VITRO ACTIVITY ; PARENTERAL CARBAPENEM ; HEALTHY-VOLUNTEERS ; RAPID-DETERMINATION ; DEHYDROPEPTIDASE-I ; STABILITY ; ERTAPENEM ; FLUID
英文摘要:

Existing methods to determine biapenem (CAS 120410-24-4), a carbapenem, either lacked sensitivity/reproducibility or had no internal standard as a control. Here an improved reversed-phase high-performance liquid chromatographic (RP-HPLC) method was established in human plasma and urine. After adding p-aminobenzoic acid as the internal standard to plasma or urine, plasma samples were ultra-filtrated and urine samples were diluted directly. Chromatographic separations were carried out on a 4.6 mm x 150 mm column with acetonitrile-0.1 mol/l sodium acetate (2:98, v:v; pH 4.38 or 4.00) as mobile phase and UV detection at 300 nm. The extraction recovery was 91.51% for biapenem at the concentration level of 5 mu g /ml in human plasma. The linear quantification range of the method was 0.1 similar to 50 mu g /ml for plasma and urine, with linear correlation coefficients greater than 0.998. The intra-day and inter-day relative standard deviations (R.S.D.) for biapenem at low, middle and high levels in human samples were less than 12.51% for plasma and less than 7.05% for urine. The RP-HPLC method was successfully applied to pharmacokinetic studies, in which healthy subjects received multiple doses of biapenem (300 mg, i. v., b. i. d., for 5 continuous days). The pharmacokinetic results are presented.

语种: 英语
WOS记录号: WOS:000289130600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57752
Appears in Collections:北京大学第二临床医学院_药剂科_期刊论文

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作者单位: Beijing Univ, Dept Pharm, Peoples Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Zhao, Liho,Liu, Yi,Kou, Zhibin,et al. Improved RP-HPLC method to determine biapenem in human plasma/urine and its application to a pharmacokinetic study[J]. ARZNEIMITTELFORSCHUNG-DRUG RESEARCH,2011,61(3):197-204.
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