IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Adiponectin Increases Secretion of Rat Submandibular Gland via Adiponectin Receptors-Mediated AMPK Signaling
Ding, Chong1,2; Li, Li3,4; Su, Yun-Chao3,4; Xiang, Ruo-Lan3,4; Cong, Xin3,4; Yu, Hong-Kui5; Li, Sheng-Lin1,2; Wu, Li-Ling3,4; Yu, Guang-Yan5
刊名PLOS ONE
2013-05-07
DOI10.1371/journal.pone.0063878
8期:5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ACTIVATED PROTEIN-KINASE ; EPITHELIAL TIGHT JUNCTIONS ; MYOCARDIAL-INFARCTION ; CELL MONOLAYERS ; DIABETIC-RATS ; EXPRESSION ; SALIVARY ; ASSOCIATION ; HYPOADIPONECTINEMIA ; PHOSPHORYLATION
英文摘要

Adiponectin and adiponectin receptors (AdipoR1/2) are expressed in various tissues and are involved in the regulation of multiple functions such as energy metabolism and inflammatory responses. However, the effect of adiponectin and AdipoRs in submandibular glands has not been fully evaluated. In the present study, we found that mRNA and protein of both adiponectin and AdipoR1/2 were expressed in rat submandibular glands and in the SMG-C6 cell line, as evidenced by RT-PCR and Western blot analysis. Immunofluorescence staining showed that adiponectin was diffused in the cytoplasm, while AdipoR1/2 was concentrated in the membrane of acinar cells. Saliva flow was significantly increased by full length adiponectin (fAd) or globular adiponectin (gAd) perfusion in isolated rat submandibular glands. 5-Aminoimidazole-4carboxamide- 1-4-ribofuranoside (AICAR), an adenosine monophosphate activated protein kinase (AMPK) activator, also increased saliva secretion. fAd, gAd, and AICAR all increased the average width of apical tight junctions in perfused submandibular glands, and decreased transepithelial electrical resistance (TER) in SMG-C6 cells, suggesting that adiponectin promoted secretion by modulating paracellular permeability. fAd and gAd increased p-AMPK levels, while AraA, an AMPK antagonist, abolished fAd- and gAd-induced changes in secretion, tight junction ultrastructure, and TER. Moreover, both AdipoR1 and AdipoR2 were required for fAd- or gAd-induced p-AMPK and TER responses, suggesting from their inhibition following AdipoR1 or AdipoR2 knockdown, and co-knockdown of AdipoRs by RNA interference. Our results suggest that adiponectin functions as a promoter of salivary secretion in rat submandibular glands via activation of AdipoRs, AMPK, and paracellular permeability.

语种英语
WOS记录号WOS:000319654700193
项目编号30973316 ; 81070847 ; 81271161
资助机构National Natural Science Foundation of China
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57753
专题北京大学口腔医学院
北京大学基础医学院
北京大学口腔医学院_外科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Ctr Salivary Gland Dis, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Lab, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100871, Peoples R China
4.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Ding, Chong,Li, Li,Su, Yun-Chao,et al. Adiponectin Increases Secretion of Rat Submandibular Gland via Adiponectin Receptors-Mediated AMPK Signaling[J]. PLOS ONE,2013,8(5).
APA Ding, Chong.,Li, Li.,Su, Yun-Chao.,Xiang, Ruo-Lan.,Cong, Xin.,...&Yu, Guang-Yan.(2013).Adiponectin Increases Secretion of Rat Submandibular Gland via Adiponectin Receptors-Mediated AMPK Signaling.PLOS ONE,8(5).
MLA Ding, Chong,et al."Adiponectin Increases Secretion of Rat Submandibular Gland via Adiponectin Receptors-Mediated AMPK Signaling".PLOS ONE 8.5(2013).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ding, Chong]的文章
[Li, Li]的文章
[Su, Yun-Chao]的文章
百度学术
百度学术中相似的文章
[Ding, Chong]的文章
[Li, Li]的文章
[Su, Yun-Chao]的文章
必应学术
必应学术中相似的文章
[Ding, Chong]的文章
[Li, Li]的文章
[Su, Yun-Chao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。