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学科主题: 基础医学
题名:
In vitro and in vivo induction of bone formation based on ex vivo gene therapy using rat adipose-derived adult stem cells expressing BMP-7
作者: Yang, M; Ma, QJ; Dang, GT; Ma, KT; Chen, P; Zhou, CY
关键词: adipose-derived adult stem cells ; BMP-7 ; differentiation ; gene therapy
刊名: CYTOTHERAPY
发表日期: 2005-07-01
DOI: 10.1080/14653240510027244
卷: 7, 期:3, 页:273-281
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology ; Hematology ; Medicine, Research & Experimental
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Hematology ; Research & Experimental Medicine
关键词[WOS]: ADIPOCYTIC DIFFERENTIATION ; STROMAL CELLS ; TISSUE ; FAT ; REGENERATION ; PROTEINS ; DEFECTS ; REPAIR
英文摘要:

Background Adipose-derived adult stem (ADAS) cells are multipotent cells capable of differentiating into osteoblasts, adipocytes and chondrocytes. The aim of this study was to determine whether BMP-7-expressing ADAS cells would elicit bone formation in vitro and in vivo.

Methods ADAS cells were harvested from Lewis rats and transduced with adenovirus carrying the recombinant human bone morphogenetic protein-7 (Ad-BMP-7) gene. Untransduced cells and cells transduced with adenovirus carrying the enhanced green fluorescence protein (Ad-EGFP) gene served as controls. BMP-7 expression was assessed by RT-PCR, immunofluorescence on day 1, and Western blot on days 4, 8 and 12. Alkaline phosphatase (ALP) activity was assayed on days 2, 4, 6, 8, 10 and 12. Osteocalcin production and bone nodule formation were detected by immunohistochemistry and von Kossa stain on day 12. A total of 1 x 10 6 cells mixed with type I collagen were implanted into the subcutaneous pocket in Lewis rat and subjected to histologic analysis 1, 2 and 4 weeks post-implantation.

Results The Ad-BMP-7-transduced ADAS cells expressed BMP-7 at both mRNA and protein levels. ALP activity was detected in Ad-BMP-7-transduced cells from day 2 to day 12, peaking on day 8. Osteocalcin production and matrix mineralization further confirmed that these cells differentiated into osteoblasts and induced bone formation in vitro. Histologic examination revealed that implantation of BMP-7-expressing ADAS cells could induce new bone formation in vivo.

Discussion ADAS cells would be a promising source of adult autologous stem cells for BMP gene therapy and tissue engineering.

语种: 英语
WOS记录号: WOS:000230988200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57756
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100871, Peoples R China

Recommended Citation:
Yang, M,Ma, QJ,Dang, GT,et al. In vitro and in vivo induction of bone formation based on ex vivo gene therapy using rat adipose-derived adult stem cells expressing BMP-7[J]. CYTOTHERAPY,2005,7(3):273-281.
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