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IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha(1)-adrenoceptor subtypes in HEK293 cells
作者: Lei, BL; Zhang, YY; Han, CD
关键词: alpha(1)-adrenoceptor ; mRNA expression ; noradrenaline ; regulation ; signal transduction
刊名: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
发表日期: 2002-12-01
卷: 29, 期:12, 页:1084-1090
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Physiology
研究领域[WOS]: Pharmacology & Pharmacy ; Physiology
关键词[WOS]: VASCULAR SMOOTH-MUSCLE ; RECEPTOR MESSENGER-RNA ; ALPHA-1-ADRENERGIC RECEPTOR ; MEDIATED REGULATION ; MOLECULAR-CLONING ; DOWN-REGULATION ; CDNA ; AGONIST
英文摘要:

1. The aim of the present study was to investigate noradrenaline (NA)-induced regulation of alpha(1) -adrenoceptor (AR) mRNA expression in human embryonic kidney (HEK) 293 cells stably expressing cloned alpha(1) -AR subtypes with similar receptor densities. Stable transfection was performed by calcium phosphate precipitation. Receptor expression was detected by radioligand binding assay. The mRNA expression was measured by RNase protection assay.

2. alpha(1) -Adrenoceptor subtype mRNA respond in distinct ways following prolonged exposure to NA. The mRNA level of the alpha(1A) -AR subtype was unchanged, the mRNA level of the alpha(1B) -AR subtype was increased and the mRNA level of the alpha(1D) -AR subtype declined time dependently. The protein kinase C (PKC) inhibitor calphostin C or RO 31-8220 abolished the NA-induced downregulation of alpha(1D) -AR mRNA. Phorbol myristate acetate (PMA), a PKC activator, similarly repressed the effects of NA on alpha(1D) -AR. However, calphostin C, RO 31-8220 or PMA had no effect on the induction of alpha(1B) -AR mRNA by NA. The Ca2+ -ATPase inhibitor thapsigargin or the calcium chelator 1,2-bis-(o -aminophenoxy) ethane-N ,N ,N ′,N ′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM) had no effect on the repression of alpha(1D) -AR mRNA, but did inhibit the induction of alpha(1B) -AR mRNA by NA. Noradrenaline significantly decelerated the degradation of alpha(1B) -AR mRNA, but had no effect on the degradation of alpha(1D) -AR mRNA.

3. Thus, the mRNA expression of three alpha(1) -AR subtypes in HEK293 cells is differentially regulated through distinct signal transduction pathways under sustained NA stimulation. The upregulation of alpha(1B) -AR mRNA is via the Ca2+ pathway, whereas the downregulation of alpha(1D) -AR mRNA is via the PKC pathway.

语种: 英语
WOS记录号: WOS:000178674800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57764
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Educ Mol Cardiovasol, Reference Lab, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China

Recommended Citation:
Lei, BL,Zhang, YY,Han, CD. Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha(1)-adrenoceptor subtypes in HEK293 cells[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2002,29(12):1084-1090.
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