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学科主题临床医学
Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha(1)-adrenoceptor subtypes in HEK293 cells
Lei, BL; Zhang, YY; Han, CD
关键词Alpha(1)-adrenoceptor Mrna Expression Noradrenaline Regulation Signal Transduction
刊名CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
2002-12-01
29期:12页:1084-1090
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Physiology
研究领域[WOS]Pharmacology & Pharmacy ; Physiology
关键词[WOS]VASCULAR SMOOTH-MUSCLE ; RECEPTOR MESSENGER-RNA ; ALPHA-1-ADRENERGIC RECEPTOR ; MEDIATED REGULATION ; MOLECULAR-CLONING ; DOWN-REGULATION ; CDNA ; AGONIST
英文摘要

1. The aim of the present study was to investigate noradrenaline (NA)-induced regulation of alpha(1) -adrenoceptor (AR) mRNA expression in human embryonic kidney (HEK) 293 cells stably expressing cloned alpha(1) -AR subtypes with similar receptor densities. Stable transfection was performed by calcium phosphate precipitation. Receptor expression was detected by radioligand binding assay. The mRNA expression was measured by RNase protection assay.

2. alpha(1) -Adrenoceptor subtype mRNA respond in distinct ways following prolonged exposure to NA. The mRNA level of the alpha(1A) -AR subtype was unchanged, the mRNA level of the alpha(1B) -AR subtype was increased and the mRNA level of the alpha(1D) -AR subtype declined time dependently. The protein kinase C (PKC) inhibitor calphostin C or RO 31-8220 abolished the NA-induced downregulation of alpha(1D) -AR mRNA. Phorbol myristate acetate (PMA), a PKC activator, similarly repressed the effects of NA on alpha(1D) -AR. However, calphostin C, RO 31-8220 or PMA had no effect on the induction of alpha(1B) -AR mRNA by NA. The Ca2+ -ATPase inhibitor thapsigargin or the calcium chelator 1,2-bis-(o -aminophenoxy) ethane-N ,N ,N ′,N ′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM) had no effect on the repression of alpha(1D) -AR mRNA, but did inhibit the induction of alpha(1B) -AR mRNA by NA. Noradrenaline significantly decelerated the degradation of alpha(1B) -AR mRNA, but had no effect on the degradation of alpha(1D) -AR mRNA.

3. Thus, the mRNA expression of three alpha(1) -AR subtypes in HEK293 cells is differentially regulated through distinct signal transduction pathways under sustained NA stimulation. The upregulation of alpha(1B) -AR mRNA is via the Ca2+ pathway, whereas the downregulation of alpha(1D) -AR mRNA is via the PKC pathway.

语种英语
WOS记录号WOS:000178674800006
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57764
专题北京大学第三临床医学院_心血管内科
作者单位1.Minist Educ Mol Cardiovasol, Reference Lab, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
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Lei, BL,Zhang, YY,Han, CD. Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha(1)-adrenoceptor subtypes in HEK293 cells[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2002,29(12):1084-1090.
APA Lei, BL,Zhang, YY,&Han, CD.(2002).Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha(1)-adrenoceptor subtypes in HEK293 cells.CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,29(12),1084-1090.
MLA Lei, BL,et al."Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha(1)-adrenoceptor subtypes in HEK293 cells".CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 29.12(2002):1084-1090.
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