北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro
作者: Wang Runchao; Wan Zhe; Li Ruoyu
关键词: dendritic cells ; Aspergillus fumigatus ; helper T cells ; regulatory T cells
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2014-10-20
DOI: 10.3760/cma.j.issn.0366-6999.20141433
卷: 127, 期:20, 页:3616-3622
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS ; INVASIVE ASPERGILLOSIS ; TRANSPLANT RECIPIENTS ; FUNGAL-INFECTIONS ; CYSTIC-FIBROSIS ; IMMUNITY ; VACCINATION ; GENERATION ; ANTIGENS ; CONIDIA
英文摘要:

Background Dendritic cells (DCs) can recognize the pathogen-associated molecular patterns (PAMP) of Aspergillus fumigatus (A. fumigatus), activating the immune response. During A. fumigatus infection, a Th and Treg response induced in the fungi-pulsed DCs is not yet well understood.

Methods In this study, bone marrow-derived dendritic cells (BMDCs) were separated and proliferated from C57BU6 mice. A. fumigatus pulsed DCs were generated and cultured with CD4(+) T cells derived from the spleen of C57BL/6 mice in vitro. CD4+ T cells differentiation after co-culture were analyzed by flow cytometry, ELISA, and real-time PCR analysis.

Results The A. fumigatus pulsed DCs exhibited increased Th1 and Treg frequency, Th1-related cytokines (IFN-gamma and IL12), Treg-related cytokines (TGF-beta) and T-bet, and Foxp3 mRNA levels compared with the control group. There was no significant difference between A. fumigatus pulsed DCs group and the control group about Th17 and Th2 frequency.

Conclusions The inactivated conidia of A. fumigatus were able to activate BMDCs and made them capable of triggering T cell responses in vitro. A. fumigatus loaded DCs was a weak inducer of Th17 and Th2, but induced a strong Th1 and Treg response.

语种: 英语
所属项目编号: 30930006
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000344724100018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57765
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Hosp 1, Res Ctr Med Mycol, Dept Dermatol, Beijing 100034, Peoples R China

Recommended Citation:
Wang Runchao,Wan Zhe,Li Ruoyu. Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro[J]. CHINESE MEDICAL JOURNAL,2014,127(20):3616-3622.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang Runchao]'s Articles
[Wan Zhe]'s Articles
[Li Ruoyu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang Runchao]‘s Articles
[Wan Zhe]‘s Articles
[Li Ruoyu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace