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学科主题临床医学
Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro
Wang Runchao; Wan Zhe; Li Ruoyu
关键词Dendritic Cells Aspergillus Fumigatus Helper t Cells Regulatory t Cells
刊名CHINESE MEDICAL JOURNAL
2014-10-20
DOI10.3760/cma.j.issn.0366-6999.20141433
127期:20页:3616-3622
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS ; INVASIVE ASPERGILLOSIS ; TRANSPLANT RECIPIENTS ; FUNGAL-INFECTIONS ; CYSTIC-FIBROSIS ; IMMUNITY ; VACCINATION ; GENERATION ; ANTIGENS ; CONIDIA
英文摘要

Background Dendritic cells (DCs) can recognize the pathogen-associated molecular patterns (PAMP) of Aspergillus fumigatus (A. fumigatus), activating the immune response. During A. fumigatus infection, a Th and Treg response induced in the fungi-pulsed DCs is not yet well understood.

Methods In this study, bone marrow-derived dendritic cells (BMDCs) were separated and proliferated from C57BU6 mice. A. fumigatus pulsed DCs were generated and cultured with CD4(+) T cells derived from the spleen of C57BL/6 mice in vitro. CD4+ T cells differentiation after co-culture were analyzed by flow cytometry, ELISA, and real-time PCR analysis.

Results The A. fumigatus pulsed DCs exhibited increased Th1 and Treg frequency, Th1-related cytokines (IFN-gamma and IL12), Treg-related cytokines (TGF-beta) and T-bet, and Foxp3 mRNA levels compared with the control group. There was no significant difference between A. fumigatus pulsed DCs group and the control group about Th17 and Th2 frequency.

Conclusions The inactivated conidia of A. fumigatus were able to activate BMDCs and made them capable of triggering T cell responses in vitro. A. fumigatus loaded DCs was a weak inducer of Th17 and Th2, but induced a strong Th1 and Treg response.

语种英语
WOS记录号WOS:000344724100018
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57765
专题北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Hosp 1, Res Ctr Med Mycol, Dept Dermatol, Beijing 100034, Peoples R China
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Wang Runchao,Wan Zhe,Li Ruoyu. Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro[J]. CHINESE MEDICAL JOURNAL,2014,127(20):3616-3622.
APA Wang Runchao,Wan Zhe,&Li Ruoyu.(2014).Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro.CHINESE MEDICAL JOURNAL,127(20),3616-3622.
MLA Wang Runchao,et al."Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro".CHINESE MEDICAL JOURNAL 127.20(2014):3616-3622.
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