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学科主题: 药学
题名:
Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry
作者: Qiao, Xue1; Song, Wei1; Ji, Shuai1; Wang, Qi1; Guo, De-an1,2; Ye, Min1,2
关键词: 2DLC ; Licorice ; Glycyrrhiza uralensis ; Phenolic compounds ; Triterpenoid saponins
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2015-07-10
DOI: 10.1016/j.chroma.2015.05.006
卷: 1402, 页:36-45
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PRENYLATED FLAVONOIDS ; ACTIVE COMPONENTS ; CONSTITUENTS ; ROOTS ; 2ND-DIMENSION ; GLABRA ; ASSAY
英文摘要:

Licorice is one of the most popular herbal medicines worldwide. It contains a big array of phenolic compounds (flavonoids, coumarins, and diphenylethanones). Due to high structural diversity, low abundance, and co-elution with licorice saponins, these phenolic compounds are difficult to be separated by conventional chromatography. In this study, a mobile phase-dependent reversed-phase x reversed phase comprehensive two-dimensional liquid chromatography (RP x RP 2DLC) method was established to separate phenolic compounds in licorice (the roots of Glycyrrhiza uralensis). Organic solvents in the mobile phase were optimized to improve orthogonality of the first and second dimensions, and a synchronized gradient mode was used to improve chromatographic resolution. Finally, licorice extracts were eluted with methanol/water/formic acid in the first dimension (Acquity CSH C18 column), and acetonitrile/water/formic acid in the second dimension (Poroshell Phenyl-Hexyl column). By using this 2DLC system, a total of 311 compounds were detected within 40 min. The practical and effective peak capacity was 1329 and 524, respectively, and the orthogonality was 79.8%. The structures of 21 selected unknown compounds were tentatively characterized by mass spectrometry, and 8 of them were discovered from G. uralensis for the first time. The mobile phase-dependent 2DLC/MS system could benefit the separation and characterization of natural products in complicated herbal extracts. (C) 2015 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81222054 ; 81470172 ; NCET-11-0019 ; SIMM1403KF-16
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education ; State Key Laboratory of Drug Research
WOS记录号: WOS:000356979900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57769
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China

Recommended Citation:
Qiao, Xue,Song, Wei,Ji, Shuai,et al. Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1402:36-45.
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