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Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry
Qiao, Xue1; Song, Wei1; Ji, Shuai1; Wang, Qi1; Guo, De-an1,2; Ye, Min1,2
关键词2dlc Licorice Glycyrrhiza Uralensis Phenolic Compounds Triterpenoid Saponins
刊名JOURNAL OF CHROMATOGRAPHY A
2015-07-10
DOI10.1016/j.chroma.2015.05.006
1402期:0页:36-45
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]Prenylated Flavonoids ; Active Components ; Constituents ; Roots ; 2nd-dimension ; Glabra ; Assay
英文摘要

Licorice is one of the most popular herbal medicines worldwide. It contains a big array of phenolic compounds (flavonoids, coumarins, and diphenylethanones). Due to high structural diversity, low abundance, and co-elution with licorice saponins, these phenolic compounds are difficult to be separated by conventional chromatography. In this study, a mobile phase-dependent reversed-phase x reversed phase comprehensive two-dimensional liquid chromatography (RP x RP 2DLC) method was established to separate phenolic compounds in licorice (the roots of Glycyrrhiza uralensis). Organic solvents in the mobile phase were optimized to improve orthogonality of the first and second dimensions, and a synchronized gradient mode was used to improve chromatographic resolution. Finally, licorice extracts were eluted with methanol/water/formic acid in the first dimension (Acquity CSH C18 column), and acetonitrile/water/formic acid in the second dimension (Poroshell Phenyl-Hexyl column). By using this 2DLC system, a total of 311 compounds were detected within 40 min. The practical and effective peak capacity was 1329 and 524, respectively, and the orthogonality was 79.8%. The structures of 21 selected unknown compounds were tentatively characterized by mass spectrometry, and 8 of them were discovered from G. uralensis for the first time. The mobile phase-dependent 2DLC/MS system could benefit the separation and characterization of natural products in complicated herbal extracts. (C) 2015 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000356979900005
通讯作者邮箱yemin@bjmu.edu.cn
项目编号81222054 ; 81470172 ; NCET-11-0019 ; SIMM1403KF-16
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education ; State Key Laboratory of Drug Research
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57769
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学第三临床医学院_护理部
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Qiao, Xue,Song, Wei,Ji, Shuai,et al. Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1402(0):36-45.
APA Qiao, Xue,Song, Wei,Ji, Shuai,Wang, Qi,Guo, De-an,&Ye, Min.(2015).Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1402(0),36-45.
MLA Qiao, Xue,et al."Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1402.0(2015):36-45.
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