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学科主题: 临床医学
题名:
Suppression of early experimental osteoarthritis by gene transfer of interleukin-1 receptor antagonist and interleukin-10
作者: Zhang, XL; Mao, ZB; Yu, CL
关键词: gene therapy ; osteoarthritis ; interleukin-1 receptor antagonist ; interleukin-10 ; joint
刊名: JOURNAL OF ORTHOPAEDIC RESEARCH
发表日期: 2004-07-01
DOI: 10.1016/j.orthres.2003.12.007
卷: 22, 期:4, 页:742-750
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Orthopedics
研究领域[WOS]: Orthopedics
关键词[WOS]: NECROSIS-FACTOR-ALPHA ; COLLAGEN-INDUCED ARTHRITIS ; IN-VIVO ; SYNOVIAL FIBROBLASTS ; RABBIT KNEES ; VIRAL IL-10 ; THERAPY ; CARTILAGE ; EXPRESSION ; PROTEOGLYCAN
英文摘要:

Gene therapy offers a radically different approach to the treatment of arthritis. We demonstrated that cDNA coding for human interleukin-1 receptor-antagonist protein (IL-1Ra) and cDNA coding for human interleukin-10 (IL-10) can be delivered, by ex vivo techniques, to the synovial lining of joints, intra-articular expression of gene significantly reduced cartilage matrix degradation and cartilage breakdown. To achieve this, lapine synoviocytes were first transduced in culture by retroviral infection. The genetically modified synoviocytes were then transplanted by intra-articular injection into the knee joints of OA rabbits, assay of joint lavages confirmed that the gene expression was not lost 14 days after transfer. Knees receiving the lL-1Ra gene had significantly reduced cartilage breakdown. Delivery of the IL-10 gene was less effective, having only a moderate effect on cartilage breakdown. When both genes were injected together, there was a greater inhibition of cartilage breakdown, suggesting that simultaneous gene delivery may be necessary to treat OA by targeting the activities of multiple inflammatory effectors. (C) 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000222402000008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57774
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, XL,Mao, ZB,Yu, CL. Suppression of early experimental osteoarthritis by gene transfer of interleukin-1 receptor antagonist and interleukin-10[J]. JOURNAL OF ORTHOPAEDIC RESEARCH,2004,22(4):742-750.
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