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The effect of poly (L-lactic acid) nanofiber orientation on osteogenic responses of human osteoblast-like MG63 cells
Wang, Bo2,3,4; Cai, Qing1; Zhang, Shen1; Yang, Xiaoping1; Deng, Xuliang2,3,4
关键词Electrospinning Fiber Alignment Morphology Differentiation
刊名JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
2011-05-01
DOI10.1016/j.jmbbm.2011.01.008
4期:4页:600-609
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]FIBER DIAMETER ; IN-VITRO ; EXTRACELLULAR-MATRIX ; POLY(LACTIC ACID) ; ELECTROSPUN ; PROLIFERATION ; SCAFFOLDS ; DIFFERENTIATION ; MORPHOLOGY ; ALIGNMENT
英文摘要

In this study, poly (L-lactic acid) (PLLA)/trifluoroethanol (TFE) solution was electrospun to fabricate fibrous scaffolds with different fiber orientations. Random and parallel PLLA nanofiber alignments were achieved by using a metal plate and a rolling rod as the receiver, respectively. The parallel PLLA fibrous scaffolds were further hot-stretched to obtain hyperparallel PLLA fibrous scaffolds. The PLLA fibrous scaffolds were characterized by fiber diameter, interfiber distance, fiber array angle, water contact angle, morphology and mechanical strength. The tensile strength of hyperparallel nano-fibers was approximately 5- and 14-times the parallel and random fibers, respectively. Osteoblast-like MG63 cells were cultured on the PLLA scaffolds to study the effects of fiber orientation on cell morphology, proliferation and differentiation. The cells on the randomly-oriented scaffolds showed irregular forms, while the cells exhibited shuttle-like shapes on the parallel scaffolds and had larger aspect ratios along the fiber direction of the hyperparallel scaffolds. Alkaline phosphatase (ALP) activity and collagen I (placeStateCol I) and osteocalcin (OC) deposition exhibited fiber orientation dependence. With an increase in parallelism of the fibers, there was a decrease in ALP activity and placeStateCol I and OC production. These results suggest that exploitation of PLLA fiber orientation may be used to control osteoblast-like cell responses. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000289544000010
项目编号50873012 ; 2007 DFA30690
资助机构National Natural Science Foundation of China ; International Science and Technology Cooperation Program ; Program of New Century Excellent Talents (NCET) of Universities in China
引用统计
被引频次:62[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57778
专题北京大学口腔医学院
北京大学口腔医学院_特诊科
作者单位1.Beijing Univ Chem Technol, Key Lab Carbon Fiber & Funct Polymers, Minist Educ, Coll Mat Sci & Engn, Beijing 100029, Peoples R China
2.Peking Univ, Dept Geriatr Dent, Sch Stomatol, Beijing 100081, Peoples R China
3.Peking Univ, Dept Geriatr Dent, Hosp Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Ctr Biomed Mat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
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GB/T 7714
Wang, Bo,Cai, Qing,Zhang, Shen,et al. The effect of poly (L-lactic acid) nanofiber orientation on osteogenic responses of human osteoblast-like MG63 cells[J]. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS,2011,4(4):600-609.
APA Wang, Bo,Cai, Qing,Zhang, Shen,Yang, Xiaoping,&Deng, Xuliang.(2011).The effect of poly (L-lactic acid) nanofiber orientation on osteogenic responses of human osteoblast-like MG63 cells.JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS,4(4),600-609.
MLA Wang, Bo,et al."The effect of poly (L-lactic acid) nanofiber orientation on osteogenic responses of human osteoblast-like MG63 cells".JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS 4.4(2011):600-609.
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