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学科主题: 临床医学
题名:
Expression of microRNA-122 contributes to apoptosis in H9C2 myocytes
作者: Huang, Xiaoyan; Huang, Fang; Yang, Deye1; Dong, Fengquan; Shi, Xiangxiang; Wang, Hongyu3; Zhou, Xi; Wang, Suyun; Dai, Shengchuan2
关键词: miR-122 ; differential expression ; ventricular septum defect ; apoptosis ; HAND2
刊名: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
发表日期: 2012-11-01
DOI: 10.1111/j.1582-4934.2012.01577.x
卷: 16, 期:11, 页:2637-2646
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Medicine, Research & Experimental
研究领域[WOS]: Cell Biology ; Research & Experimental Medicine
关键词[WOS]: CONGENITAL HEART-DISEASE ; TRANSCRIPTION FACTOR ; CANCER-CELLS ; HAND2 ; GENE ; RNA ; SENSITIVITY ; DEFECTS ; MIRNAS ; DHAND
英文摘要:

The microRNAs (miRNAs) can post-transcriptionally regulate gene expression and heart development. The Pax-8 gene knockout mice have apparent heart abnormalities. This study investigated the role of miRNAs in regulation of cardiac apoptosis and development in the knockout mice. MicroRNA microarrays demonstrated differential expression of microRNAs between Pax-8-/- and Pax-8+/- mice, confirmed by real-time PCR. The miR-122 was up-regulated by 1.92 folds in Pax-8-/- mice. There were ventricular septum defects in Pax-8-/- mice, and increased numbers of apoptotic cells in the left ventricular wall and interventricular septum in Pax-8-/- mice. In H9C2 myocytes, treatment with miR-122 mimics or miR-122 inhibitor affects the expression of CCK-8 and activity of Caspase-3. The miR-122 is up-regulated in the myocytes of Pax-8-/- mice and may participate in the apoptotic gene expression and pathogenesis of heart development defect.

语种: 英语
所属项目编号: 30571050 ; Y2110112
项目资助者: National Natural Science Foundation of China ; Zhejiang Province Natural Science Foundation
WOS记录号: WOS:000310555200008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57782
Appears in Collections:北京大学首钢医院_心血管内科_期刊论文

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作者单位: 1.Wenzhou Med Coll, Inst Cardiovasc Biol & Gene, Affiliated Hosp 1, Div Cardiol, Wenzhou 325000, Peoples R China
2.Univ Pittsburgh, Med Ctr, Cardiovasc Inst, Div Cardiol, Pittsburgh, PA USA
3.Peking Univ, Shougang Hosp, Dept Vasc Med, Beijing 100871, Peoples R China

Recommended Citation:
Huang, Xiaoyan,Huang, Fang,Yang, Deye,et al. Expression of microRNA-122 contributes to apoptosis in H9C2 myocytes[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2012,16(11):2637-2646.
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