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IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in human gastric cancer cells
作者: Li, Y; Lu, YY
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2002-04-01
卷: 8, 期:2, 页:213-216
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: PROTEASE NEXIN-1 ; THROMBIN ; EXPRESSION ; MUSCLE ; RAT ; IDENTIFICATION ; REGENERATION ; PROTHROMBIN ; REPLICATION ; CARCINOMA
英文摘要:

AIM: To develop and optimize cDNA representational difference analysis (cDNA RDA) method and to identify and clone garlic up-regulated genes in human gastric cancer (HGC) cells.

METHODS: We performed cDNA RDA method by using abundant double-stranded cDNA messages provided by two self-constructed cDNA libraries ( Allitridi-treated and paternal HGC cell line BGC823 cells cDNA libraries respectively), BamH I and Xho I restriction sites harbored in the library vector were used to select representations. Northern and Slot blots analyses were employed to identify the obtained difference products.

RESULTS: Fragments released from the cDNA library vector after restriction endonuclease digestion acted as good marker indicating the appropriate digestion degree for library DNA. Two novel expressed sequence tags ( ESTs) and a recombinant gene were obtained. Slot blots result showed a 8-fold increase of glia-derived nexin/protease nexin I (GDN/ FIN I) gene expression level and 4-fold increase of hepatitis B virus x-interacting protein (XIP) mRNA level in BGC823 cells after Allitridi treatment for 72 h.

CONCLUSION: Elevated levels of GDN/PN1 and XIP mRNAs induced by Allitridi provide valuable molecular evidence for elucidating the garlic′s efficacies against neurodegenerative and inflammatory diseases, Isolation of a recombinant gene and two novel ESTs further show cDNA RDA based on cDNA libraries to be a powerful method with high specificity and reproducibility in cloning differentially expressed genes.

语种: 英语
WOS记录号: WOS:000175104600005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57794
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: Peking Univ, Sch Oncol, Beijing Lab Mol Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China

Recommended Citation:
Li, Y,Lu, YY. Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in human gastric cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(2):213-216.
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