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学科主题: 临床医学
题名:
Altered phospholamban-calcium ATPase interaction in cardiac sarcoplasmic reticulum during the progression of sepsis
作者: Wu, LL; Tang, CS; Dong, LW; Liu, MS
关键词: calcium homeostasis ; calcium uptake ; phospholamban phosphorylation ; cardiac depression ; shock
刊名: SHOCK
发表日期: 2002-05-01
卷: 17, 期:5, 页:389-393
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Critical Care Medicine ; Hematology ; Surgery ; Peripheral Vascular Disease
研究领域[WOS]: General & Internal Medicine ; Hematology ; Surgery ; Cardiovascular System & Cardiology
关键词[WOS]: HEART-FAILURE ; GENE-EXPRESSION ; HYPODYNAMIC PHASE ; PROTEIN-STRUCTURE ; SEPTIC SHOCK ; PHOSPHORYLATION ; HYPERTROPHY ; STIMULATION ; RAT ; TRANSITION
英文摘要:

The purpose of this study was to investigate alterations of phospholamban phosphorylation and its interaction with Ca2+ transport (Ca2+-ATPase activity and Ca2+ uptake) in sarcoplasmic reticulum (SR) during the progression of sepsis. Sepsis was induced by cecal ligation and puncture (CLIP). Phospholamban phosphorylation was studied by the labeling of the myocardial ATIP pool by perfusing isolated rat hearts with [P-32]H3PO4 followed by identification of the phosphorylated phospholamban. Results show that phospholamban phosphorylation was increased by 153% during the early hyperdynamic phase (9 h after CLIP), while it was decreased by 51% during the late hypodynamic phase (18 h after CLP) of sepsis. The increase in phospholamban phosphorylation during early sepsis was associated with increases in +dP/dt(max) and tissue cAMP content, while Ca2+ transport, left ventricular developed pressure (LVDP), and -dP/dt(max) remained unchanged. The decrease in phospholamban phosphorylation during late sepsis was accompanied by decreases in Ca2+ transport, LVDP, +/-dP/dt(max) and tissue cAMP content. When isoproterenol was present in the perfusion medium, all parameters measured were stimulated in all three experimental groups (control, early sepsis, and late sepsis) except that Ca2+-ATPase activity and SR Ca2+ Uptake were unresponsive in the early and the late septic groups. These findings demonstrate that during the late hypodynamic phase of sepsis, the observed decrease in myocardial contractility was due to the decrease in phospholamban,phosphorylation, which resulted in decreased Ca2+ transport across the SR. In contrast, during the early hyperdynamic phase of sepsis, the increase in phospholamban phosphorylation did not correlate with increases in Ca2+ uptake and Ca2+-ATPase activity. Thus, the interaction between phospholamban phosphorylation and Ca2+ transport across the SR was disrupted during the early phase of sepsis.

语种: 英语
WOS记录号: WOS:000179877100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57795
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.St Louis Univ, Sch Med, Dept Pharmacol & Physiol Sci, St Louis, MO 63104 USA
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100871, Peoples R China

Recommended Citation:
Wu, LL,Tang, CS,Dong, LW,et al. Altered phospholamban-calcium ATPase interaction in cardiac sarcoplasmic reticulum during the progression of sepsis[J]. SHOCK,2002,17(5):389-393.
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