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DNA barcoding in Mucorales: an inventory of biodiversity
Walther, G.1,2,3; Pawlowska, J.4; Alastruey-Izquierdo, A.5; Wrzosek, M.4; Rodriguez-Tudela, J. L.5; Dolatabadi, S.3,6; Chakrabarti, A.7; de Hoog, G. S.3,6,8,9
关键词Backusella Biodiversity Clinical Relevance Dna Barcoding Intraspecific Variability Its Lsu Mucor Mucorales Nomenclature Rhizomucor Taxonomy Zygorhynchus
刊名PERSOONIA
2013-06-01
DOI10.3767/003158513X665070
30页:11-47
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
研究领域[WOS]Mycology
关键词[WOS]GENUS RHIZOPUS ; PHYLOGENETIC-RELATIONSHIPS ; MOLECULAR PHYLOGENY ; RDNA SEQUENCES ; SPECIES RECOGNITION ; FILAMENTOUS FUNGI ; ORDER MUCORALES ; SP-NOV ; IDENTIFICATION ; MUCORMYCOSIS
英文摘要

The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and sporangiola, traditionally used in mucoralean taxonomy are plesiomorphic traits. The ITS region turned out to be an appropriate barcoding marker in Mucorales. It could be sequenced directly in 82 % of the strains and its variability was sufficient to resolve most of the morphospecies. Molecular identification turned out to be problematic only for the species complexes of Mucor circinelloides, M. flavus, M. piriformis and Zygorhynchus moelleri. As many as 12 possibly undescribed species were detected. Intraspecific variability differed widely among mucorealean species ranging from 0 % in Backusella circina to 13.3 % in Cunninghamella echinulata. A high proportion of clinical strains was included for molecular identification. Clinical isolates of Cunninghamella elegans were identified molecularly for the first time. As a result of the phylogenetic analyses several taxonomic and nomenclatural changes became necessary. The genus Backusella was emended to include all species with transitorily recurved sporangiophores. Since this matched molecular data all Mucor species possessing this character were transferred to Backusella. The genus Zygorhynchus was shown to be polyphyletic based on ITS and LSU data. Consequently, Zygorhynchus was abandoned and all species were reclassified in Mucor. Our phylogenetic analyses showed, furthermore, that all non-thermophilic Rhizomucor species belong to Mucor. Accordingly, Rhizomucor endophyticus was transferred to Mucor and Rhizomucor chlamydosporus was synonymised with Mucor indicus. Lecto-, epi- or neotypes were designated for several taxa.

语种英语
WOS记录号WOS:000317399800003
引用统计
被引频次:92[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57817
专题北京大学第一临床医学院_皮肤性病科
作者单位1.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
2.Univ Warsaw, Dept Systemat & Plant Geog, Warsaw, Poland
3.Univ Jena, Inst Microbiol, Dept Microbiol & Mol Biol, Jena, Germany
4.Jena Microbial Resource Collect, Hans Knoll Inst, Leibniz Inst Nat Prod Res & Infect Biol, Jena, Germany
5.Spanish Natl Ctr Microbiol, Inst Salud Carlos III, Dept Mycol, Madrid, Spain
6.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
7.Postgrad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh 160012, India
8.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
9.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China
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GB/T 7714
Walther, G.,Pawlowska, J.,Alastruey-Izquierdo, A.,et al. DNA barcoding in Mucorales: an inventory of biodiversity[J]. PERSOONIA,2013,30:11-47.
APA Walther, G..,Pawlowska, J..,Alastruey-Izquierdo, A..,Wrzosek, M..,Rodriguez-Tudela, J. L..,...&de Hoog, G. S..(2013).DNA barcoding in Mucorales: an inventory of biodiversity.PERSOONIA,30,11-47.
MLA Walther, G.,et al."DNA barcoding in Mucorales: an inventory of biodiversity".PERSOONIA 30(2013):11-47.
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