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学科主题口腔医学
Effect of Bacteria on the Wound Healing Behavior of Oral Epithelial Cells
Bhattacharya, Rupa1; Xu, Fanxing1,2; Dong, Guangyu1; Li, Shuai3; Tian, Chen1; Ponugoti, Bhaskar1; Graves, Dana T.1
刊名PLOS ONE
2014-02-21
DOI10.1371/journal.pone.0089475
9期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Institute of Dental and Craniofacial Research ; National Institute of Dental and Craniofacial Research
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PORPHYROMONAS-GINGIVALIS PROTEINASES ; CYCLIN-DEPENDENT KINASES ; PERIODONTAL-DISEASE ; VIRULENCE ; PROGRESSION ; INTEGRINS ; INVASION ; REPAIR
英文摘要

Wounded tissue offers opportunity to microflora to adhere, colonize, invade and infect surrounding healthy tissue. The bacteria of the oral cavity have the potential to alter the wound healing process by interacting with keratinocytes. The aim of this study was to investigate mechanisms through which oral bacteria may influence re-epithelialization by interacting with gingival keratinocytes. By an in vitro scratch assay we demonstrate that primary gingival keratinocytes have impaired closure when exposed to two well characterized oral bacteria, P. gingivalis, and to a lesser extent, F. nucleatum. P. gingivalis reduced wound closure by similar to 40%, which was partially dependent on proteolytic activity, and bacteria was still present within infected cells 9 days later despite exposure to bacteria for only 24 h. Both oral bacteria caused keratinocyte apoptosis at the wound site with cell death being greatest at the wound edge. P. gingivalis and F. nucleatum adversely affected cell proliferation and the effect also had a spatial component being most striking at the edge. The impact of the bacteria was long lasting even when exposure was brief. Cell migration was compromised in bacteria challenged keratinocytes with P. gingivalis having more severe effect (p < 0.05) than F. nucleatum. Quantitative real time PCR of bacteria challenged cells showed that P. gingivalis and to a lesser extent F. nucleatum significantly downregulated cell cycle genes cyclin1, CDK1, and CDK4 (p < 0.05) that are critical for GI/S transition. Further, genes associated with cell migration such as integrin beta-3 and -6 were significantly downregulated by P. gingivalis (p < 0.05).

语种英语
所属项目编号DE018307 ; DE019108
资助者National Institute of Dental and Craniofacial Research ; National Institute of Dental and Craniofacial Research
WOS记录号WOS:000331717900103
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57832
专题北京大学口腔医学院_口腔种植科
作者单位1.Univ Penn, Sch Dent Med, Dept Periodont, Philadelphia, PA 19104 USA
2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Implant Dent, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Bhattacharya, Rupa,Xu, Fanxing,Dong, Guangyu,et al. Effect of Bacteria on the Wound Healing Behavior of Oral Epithelial Cells[J]. PLOS ONE,2014,9(2).
APA Bhattacharya, Rupa.,Xu, Fanxing.,Dong, Guangyu.,Li, Shuai.,Tian, Chen.,...&Graves, Dana T..(2014).Effect of Bacteria on the Wound Healing Behavior of Oral Epithelial Cells.PLOS ONE,9(2).
MLA Bhattacharya, Rupa,et al."Effect of Bacteria on the Wound Healing Behavior of Oral Epithelial Cells".PLOS ONE 9.2(2014).
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