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Functional supertype of HLA-A2 in the presentation of Flu matrix p58-66 to induce CD8(+) T-cell response in a Northern Chinese population
Zhang, HG; Pang, XW; Shang, XY; Xing, Q; Chen, WF
关键词DC Elispot assay HLA supertype HLA typing tetramer assay
刊名TISSUE ANTIGENS
2003-10-01
62期:4页:285-295
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology ; Pathology
研究领域[WOS]Cell Biology ; Immunology ; Pathology
关键词[WOS]DENDRITIC CELLS ; BINDING ; PEPTIDE ; ANTIGEN ; SUPERMOTIFS ; MOLECULES ; SUBTYPES ; IMMUNOGENICITY ; RECOGNITION ; LYMPHOCYTES
英文摘要

The functional supertype of HLA-A2 was investigated in the presentation of the A*0201-restricted Flu matrix p58-66 peptide to activate recall CD8(+) T-cell response. In healthy Northern Chinese, the HLA-A2 supertype was mainly composed of the six alleles, A*0201 (26.4%), A*0206 (12.7%), A*0203 (8.2%), A*0207 (7.3%), A*0210 (1.8%) and A*0205 (0.9%), as analyzed by PCR using sequence specific primer (PCR-SSP) and sequence based typing (SBT). The IFN-gamma release Elispot assay was employed to assess effector CD8(+) T cells. In A*0201-bearing individuals, the CD8(+) T-cell response was potent when stimulated with autologous CD8(-) PBMCs. The frequency of the effector CD8(+) T cells was 96.6% with the magnitude of effector CD8(+) T cells of 225 SFC/5 x 10(4) CD8(+) T cells and the RI of 25.7. In non-A*0201 individuals, the effector CD8(+) T cells were minimally detectable while the peptide was presented by the autologous CD8(-) PBMCs. However, the induction of the response of CD8(+) T cells obtained from non-A*0201 individuals was remarkably improved when the peptide was presented by autologous dendritic cells instead of CD8- PBMCs. The HLA-A2 alleles possessing cross-reactivity in the peptide presentation were mainly of A*0206 and non-A*0201 heterozygotes of A*0206 and A*0210. Moreover, A*0206 as the HLA-A2 functional supertype was further confirmed by tetramer assay. In two A*0206(+) donors with CD8(+) T-cell response to the peptide, the CD8(+) T-cell frequency assessed by specific binding of peptide HLA-A*0201 tetramer was 4.62% and 1.66%, respectively. Thus, our results have substantiated the immunological relevance of the HLA-A2 supertype, which may benefit the design of peptide vaccines with the potential to be applicable in broader populations.

语种英语
WOS记录号WOS:000185701700002
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57839
专题基础医学院_免疫学系
作者单位Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, HG,Pang, XW,Shang, XY,et al. Functional supertype of HLA-A2 in the presentation of Flu matrix p58-66 to induce CD8(+) T-cell response in a Northern Chinese population[J]. TISSUE ANTIGENS,2003,62(4):285-295.
APA Zhang, HG,Pang, XW,Shang, XY,Xing, Q,&Chen, WF.(2003).Functional supertype of HLA-A2 in the presentation of Flu matrix p58-66 to induce CD8(+) T-cell response in a Northern Chinese population.TISSUE ANTIGENS,62(4),285-295.
MLA Zhang, HG,et al."Functional supertype of HLA-A2 in the presentation of Flu matrix p58-66 to induce CD8(+) T-cell response in a Northern Chinese population".TISSUE ANTIGENS 62.4(2003):285-295.
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