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学科主题: 临床医学
题名:
Improved sensitivity of vaginal self-collection and high-risk human papillomavirus testing
作者: Belinson, Jerome L.1; Du, Hui2; Yang, Bin3; Wu, Ruifang2; Belinson, Suzanne E.1; Qu, Xinfeng4; Pretorius, Robert G.5; Yi, Xin6; Castle, Philip E.7
关键词: HPV ; screening ; self-collection ; cervical cancer
刊名: INTERNATIONAL JOURNAL OF CANCER
发表日期: 2012-04-15
DOI: 10.1002/ijc.26202
卷: 130, 期:8, 页:1855-1860
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CERVICAL-CANCER ; HPV HR ; TRIAL ; DETECT ; ACCURACY ; CYTOLOGY ; WOMEN
英文摘要:

Self-collected vaginal specimens tested for high-risk human papillomavirus (HR-HPV) have been shown to be less sensitive for the detection of cervical intraepithelial neoplasia or cancer (>= CIN 3) than physician-collected endocervical specimens. To increase the sensitivity of self-collected specimens, we studied a self-sampling device designed to obtain a larger specimen from the upper vagina (POI/NIH self-sampler) and a more sensitive polymerase chain reaction (PCR)-based HR-HPV assay. Women (10,000) were screened with cervical cytology and HR-HPV testing of vaginal self-collected and endocervical physician-collected specimens. Women were randomly assigned to use either a novel self-collection device (POI/NIH self-sampler) or conical-shaped brush (Qiagen). The self-collected and clinician-collected specimens were assayed by Cervista (Hologic) and the research only PCR-based matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). Women with any abnormal screening test underwent colposcopy and biopsy. Women (8,556), mean age of 38.9, had complete data; 1.6% had >= CIN 3. For either HR-HPV assay, the sensitivity was similar for the two self-collection devices. Tested with Cervista, the sensitivity for >= CIN 3 of self-collected specimens was 70.9% and for endocervical specimens was 95.0% (p = 0.0001). Tested with MALDI-TOF, the sensitivity for >= CIN 3 of self-collected specimens was 94.3% and for endocervical specimens was also 94.3% (p = 1.0). A self-collected sample using a PCR-based assay with the capability of very high throughput has similar sensitivity as a direct endocervical specimen obtained by a physician. Large population-based screening "events" in low-resource settings could be achieved by promoting self-collection and centralized high-throughput, low-cost testing by PCR-based MALDI-TOF.

语种: 英语
WOS记录号: WOS:000300692300015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57842
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Kaiser Permanente, Dept Obstet & Gynecol, Fontana, OH USA
2.BGI Shenzhen, Shenzhen, Peoples R China
3.Amer Soc Clin Pathologists, Chicago, IL USA
4.Prevent Oncol Int Inc, Cleveland Hts, OH USA
5.Peking Univ, Shenzhen Hosp, Shenzhen, Peoples R China
6.Cleveland Clin, Dept Anat Pathol, Cleveland, OH 44106 USA
7.Royal Ladies Clin, POI Res Lab, Shenzhen, Peoples R China

Recommended Citation:
Belinson, Jerome L.,Du, Hui,Yang, Bin,et al. Improved sensitivity of vaginal self-collection and high-risk human papillomavirus testing[J]. INTERNATIONAL JOURNAL OF CANCER,2012,130(8):1855-1860.
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