IR@PKUHSC  > 北京大学第二临床医学院
Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy
Li ShuLong1,2; Liu Xin1,6; Wei Lai3; Wang HuiFen4; Zhang JiYang1; Wei HanDong1; Qian XiaoHong1; Jiang Ying1; He FuChu1,5
Source PublicationSCIENCE CHINA-LIFE SCIENCES
2011-05-01
Volume54Issue:5Pages:393-402
Indexed BySCI
Abstract

A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease, treatment decisions, and assessing drug efficacy. This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging. Pooled plasma from different liver fibrosis stages, which were assessed in advance by the current gold-standard of liver biopsy, was quantitatively analyzed. A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process, and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis. Validation results of fibronectin by Western blotting reconfirmed the mass-based data. Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients. Consequently, quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis. We quantitatively monitored the fibrogenesis process in CHB patients. We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease. These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.

KeywordQuantitative Proteomics Liver Fibrosis Biomarker Plasma Hepatitis b Virus
Subject Area临床医学
SubtypeArticle
DOI10.1007/s11427-011-4165-y
WOS HeadingsScience & Technology
Language英语
Funding OrganizationNational Basic Research Program of China ; National High Technology Research and Development Program of China ; National Natural Science Foundation of China ; Chinese State Key Project Specialized for Infectious Diseases ; National Key Technologies R&amp ; D Program for New Drugs ; International Scientific Collaboration Program ; State Key Laboratory of Proteomics
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS KeywordCHRONIC HEPATITIS-C ; AMYLOID P-COMPONENT ; MASS-SPECTROMETRY ; VIRUS-INFECTION ; QUANTITATIVE PROTEOMICS ; HEPATOCELLULAR-CARCINOMA ; ALDEHYDE DEHYDROGENASE-2 ; NONINVASIVE MARKERS ; MOLECULAR ASPECTS ; DISEASE
WOS IDWOS:000292050800001
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.bjmu.edu.cn/handle/400002259/57850
Collection北京大学第二临床医学院
北京大学第二临床医学院_北京大学肝病研究所
北京大学口腔医学院_外科
Affiliation1.Beijing Inst Biotechnol, Beijing 100071, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China
3.302 Mil Hosp China, Beijing 100039, Peoples R China
4.Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China
5.Yantai Yu Huang Ding Hosp, Cent Lab, Yantai 264000, Peoples R China
6.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China
First Author AffilicationPEKING UNIVERSITY HEALTH SCIENCE CENTER
Recommended Citation
GB/T 7714
Li ShuLong,Liu Xin,Wei Lai,et al. Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(5):393-402.
APA Li ShuLong.,Liu Xin.,Wei Lai.,Wang HuiFen.,Zhang JiYang.,...&He FuChu.(2011).Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy.SCIENCE CHINA-LIFE SCIENCES,54(5),393-402.
MLA Li ShuLong,et al."Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy".SCIENCE CHINA-LIFE SCIENCES 54.5(2011):393-402.
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