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学科主题: 临床医学
题名:
Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy
作者: Li ShuLong1,2; Liu Xin1,6; Wei Lai3; Wang HuiFen4; Zhang JiYang1; Wei HanDong1; Qian XiaoHong1; Jiang Ying1; He FuChu1,5
关键词: quantitative proteomics ; liver fibrosis ; biomarker ; plasma ; hepatitis B virus
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2011-05-01
DOI: 10.1007/s11427-011-4165-y
卷: 54, 期:5, 页:393-402
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: CHRONIC HEPATITIS-C ; AMYLOID P-COMPONENT ; MASS-SPECTROMETRY ; VIRUS-INFECTION ; QUANTITATIVE PROTEOMICS ; HEPATOCELLULAR-CARCINOMA ; ALDEHYDE DEHYDROGENASE-2 ; NONINVASIVE MARKERS ; MOLECULAR ASPECTS ; DISEASE
英文摘要:

A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease, treatment decisions, and assessing drug efficacy. This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging. Pooled plasma from different liver fibrosis stages, which were assessed in advance by the current gold-standard of liver biopsy, was quantitatively analyzed. A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process, and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis. Validation results of fibronectin by Western blotting reconfirmed the mass-based data. Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients. Consequently, quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis. We quantitatively monitored the fibrogenesis process in CHB patients. We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease. These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.

语种: 英语
所属项目编号: 2011CB910601 ; 2011CB910700 ; 2010CB912700 ; 2011CB505304 ; 2006AA02A308 ; 30700356 ; 30700988 ; 30972909 ; 81000192 ; 81001470 ; 81010064 ; 2008ZX10002-016 ; 2009ZX10004-103 ; 2009ZX09301-002 ; 2009DFB33070 ; 2010DFA31260 ; SKLP-Y200901 ; SKLP-O200901
项目资助者: National Basic Research Program of China ; National High Technology Research and Development Program of China ; National Natural Science Foundation of China ; Chinese State Key Project Specialized for Infectious Diseases ; National Key Technologies R&amp ; D Program for New Drugs ; International Scientific Collaboration Program ; State Key Laboratory of Proteomics
WOS记录号: WOS:000292050800001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57850
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Beijing Inst Biotechnol, Beijing 100071, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China
3.302 Mil Hosp China, Beijing 100039, Peoples R China
4.Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China
5.Yantai Yu Huang Ding Hosp, Cent Lab, Yantai 264000, Peoples R China
6.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

Recommended Citation:
Li ShuLong,Liu Xin,Wei Lai,et al. Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(5):393-402.
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