IR@PKUHSC  > 北京大学口腔医学院  > 口腔修复科
学科主题口腔医学
Human adipose-derived adult stem cells produce osteoid in vivo
Hicok, KC; Du Laney, TV; Zhou, YS; Halvorsen, YDC; Hitt, DC; Cooper, LF; Gimble, JM
刊名TISSUE ENGINEERING
2004-03-01
10期:3-4页:371-380
收录类别ISTP ; SCI
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering
研究领域[WOS]Cell Biology
关键词[WOS]STROMAL CELLS ; OSTEOPENIC DISORDERS ; MESENCHYMAL CELLS ; PROGENITOR CELLS ; BONE ; DIFFERENTIATION ; VITRO ; GENE ; OSTEOGENESIS ; PLURIPOTENT
英文摘要

Adult subcutaneous fat tissue is an abundant source of multipotent cells. Previous studies from our laboratory have shown that, in vitro, adipose-derived adult stem (ADAS) cells express bone marker proteins including alkaline phosphatase, type I collagen, osteopontin, and osteocalcin and produce a mineralized matrix as shown by alizarin red staining. In the current study, the ADAS cell ability to form osteoid in vivo was determined. ADAS cells were isolated from liposuction waste of three individual donors and expanded in vitro before implantation. Equal numbers of cells (3 x 10(6)) were loaded onto either hydroxyapatite/tricalcium phosphate (HA-TCP) cubes or the collagen/HA-TCP composite matrix, Collagraft, and then implanted subcutaneously into SCID mice. After 6 weeks, implants were removed, fixed, and demineralized and sectioned for hematoxylin and eosin staining. Osteoid formation was observed in 80% of HA-TCP implants loaded with ADAS cells. Only 20% of Collagraft implants were positive for the presence of osteoid matrix. Whereas 100% of HA-TCP implants loaded with hFOB 1.19 cells formed osteoid, Collagraft loaded with hFOB 1.19 cells displayed a high degree of adipose tissue within the matrix. Immunostaining of serial sections for human nuclear antigen demonstrated that the osteoid contained human cells. Osteoid formation was not observed in control HA-TCP or Collagraft matrices implanted without cells. In summary, the data demonstrate the ability of ADAS cells to form osteoid matrix in vivo. Because of their abundance and accessibility, ADAS cells may prove to be a novel cell therapeutic for bone repair and regeneration.

语种英语
WOS记录号WOS:000221105000007
Citation statistics
Cited Times:223[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57860
Collection北京大学口腔医学院_口腔修复科
作者单位1.Artecel Sci, Durham, NC USA
2.UTHSC, Dept Plast Surg, San Antonio, TX USA
3.Univ N Carolina, Coll Dent, Dept Prosthodont, Chapel Hill, NC USA
4.Peking Univ, Sch Stomatol, Dept Prosthodont, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Hicok, KC,Du Laney, TV,Zhou, YS,et al. Human adipose-derived adult stem cells produce osteoid in vivo[J]. TISSUE ENGINEERING,2004,10(3-4):371-380.
APA Hicok, KC.,Du Laney, TV.,Zhou, YS.,Halvorsen, YDC.,Hitt, DC.,...&Gimble, JM.(2004).Human adipose-derived adult stem cells produce osteoid in vivo.TISSUE ENGINEERING,10(3-4),371-380.
MLA Hicok, KC,et al."Human adipose-derived adult stem cells produce osteoid in vivo".TISSUE ENGINEERING 10.3-4(2004):371-380.
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