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学科主题临床医学
Potential Localization of Putative Stem/Progenitor Cells in Human Bulbar Conjunctival Epithelium
Qi, Hong1,2; Zheng, Xiaofen1,3; Yuan, Xiaoyong1; Pflugfelder, Stephen C.1; Li, De-Quan1
刊名JOURNAL OF CELLULAR PHYSIOLOGY
2010-10-01
DOI10.1002/jcp.22215
225期:1页:180-185
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Physiology
资助者Department of Defense CDMRP PRMRP ; NIH ; National Eye Institutes, Bethesda, MD ; National Natural Science Foundation of China ; Lions Eye Bank Foundation ; Research to Prevent Blindness ; Oshman Foundation ; William Stamps Farish Fund ; Department of Defense CDMRP PRMRP ; NIH ; National Eye Institutes, Bethesda, MD ; National Natural Science Foundation of China ; Lions Eye Bank Foundation ; Research to Prevent Blindness ; Oshman Foundation ; William Stamps Farish Fund
研究领域[WOS]Cell Biology ; Physiology
关键词[WOS]NEURAL STEM-CELLS ; NEUROTROPHIC FACTORS ; HUMAN LIMBAL ; INVOLUCRIN SYNTHESIS ; OCULAR SURFACE ; GOBLET CELLS ; IN-VITRO ; EXPRESSION ; RECEPTORS ; DIFFERENTIATION
英文摘要

Although the conjunctival fornix appears to contain the greatest proportion of stem cells, it is likely that pockets of conjunctival epithelial stem cells may also exist throughout the conjunctival epithelium. This study was to investigate the potential localization of putative stem/progenitor cells in the human bulbar conjunctival epithelium by evaluating 6 keratins and 13 molecules that have been previously proposed stem cell associated or differentiation markers. We found that cornea specific cytokeratin (CK) 3 was not expressed by the bulbar conjunctival epithelial cells. In contrast, CK4 and CK7 were expressed by the superficial cells of bulbar conjunctival epithelium. CK14 and CK15 were confined to the basal cell layer. CK19 was strongly expressed by all layers of the bulbar conjunctival epithelium. The expression patterns of molecular markers in the basal cells of human bulbar conjunctival epithelium were found to be similar to the corneal epithelium. Basal conjunctival epithelial cells strongly expressed stem cell associated markers, including ABCG2, p63, nerve growth factor (NGF) with its receptors tyrosine kinase receptor A (TrkA) and neurotrophin low-affinity receptor p75NTR, glial cell-derived neurotrophic factor (GDNF) with its receptor GDNF family receptor alpha 1 (GFR alpha-1), integrin beta 1, alpha-enolase, and epidermal growth factor receptor (EGFR). The differentiation associated markers nestin, E-cadherin and involucrin were not expressed by these cells. These findings indicate that the basal cells of bulbar conjunctival epithelium shares a similar expression pattern of stem cell associated markers to the corneal epithelium, but has a unique pattern of differentiation associated cytokeratin expression. J. Cell. Physiol. 225: 180-185, 2010. (C) 2010 Wiley-Liss, Inc.

语种英语
所属项目编号FY06 PR064719 ; EY11915 ; 30872813
资助者Department of Defense CDMRP PRMRP ; NIH ; National Eye Institutes, Bethesda, MD ; National Natural Science Foundation of China ; Lions Eye Bank Foundation ; Research to Prevent Blindness ; Oshman Foundation ; William Stamps Farish Fund ; Department of Defense CDMRP PRMRP ; NIH ; National Eye Institutes, Bethesda, MD ; National Natural Science Foundation of China ; Lions Eye Bank Foundation ; Research to Prevent Blindness ; Oshman Foundation ; William Stamps Farish Fund
WOS记录号WOS:000281295100020
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57862
专题北京大学第三临床医学院_眼科
作者单位1.Baylor Coll Med, Cullen Eye Inst, Ocular Surface Ctr, Dept Ophthalmol, Houston, TX 77030 USA
2.Peking Univ, Peking Univ Third Hosp, Peking Univ Eye Ctr, Beijing 100871, Peoples R China
3.Shanxi Eye Hosp, Taiyuan, Peoples R China
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GB/T 7714
Qi, Hong,Zheng, Xiaofen,Yuan, Xiaoyong,et al. Potential Localization of Putative Stem/Progenitor Cells in Human Bulbar Conjunctival Epithelium[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2010,225(1):180-185.
APA Qi, Hong,Zheng, Xiaofen,Yuan, Xiaoyong,Pflugfelder, Stephen C.,&Li, De-Quan.(2010).Potential Localization of Putative Stem/Progenitor Cells in Human Bulbar Conjunctival Epithelium.JOURNAL OF CELLULAR PHYSIOLOGY,225(1),180-185.
MLA Qi, Hong,et al."Potential Localization of Putative Stem/Progenitor Cells in Human Bulbar Conjunctival Epithelium".JOURNAL OF CELLULAR PHYSIOLOGY 225.1(2010):180-185.
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