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学科主题临床医学
(-)-Epigallocatechin-3-gallate induces apoptosis and suppresses proliferation by inhibiting the human Indian Hedgehog pathway in human chondrosarcoma cells
Tang, Guo-Qing1; Yan, Tai-Qiang1; Guo, Wei1; Ren, Ting-Ting1; Peng, Chang-Liang1; Zhao, Hui1; Lu, Xin-Chang1; Zhao, Fu-Long1; Han, Xiaoguang2
关键词Chondrosarcoma Egcg Indian Hedgehog Pathway Apoptosis
刊名JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
2010-08-01
DOI10.1007/s00432-010-0765-3
136期:8页:1179-1185
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者Peking University People&prime ; s Hospital Research and Development Funds ; Peking University People&prime ; s Hospital Research and Development Funds
研究领域[WOS]Oncology
关键词[WOS]SIGNAL-TRANSDUCTION ; GREEN TEA ; CANCER ; DIFFERENTIATION ; SARCOMA ; NETWORK ; FAMILY ; BONE ; GENE
英文摘要

Purpose Chondrosarcoma is a soft tissue sarcoma with a poor prognosis that is unresponsive to conventional chemotherapy. The regulatory mechanisms for the rapid proliferation of chondrosarcoma cells and the particular aggressiveness of this sarcoma remain poorly understood. In this study, we investigate the effect of epigallocatechin-3-gallate (EGCG) on growth and apoptosis of chondrosarcoma cells.

Methods The chondrosarcoma cell lines, SW1353 and CRL-7891, were cultured with and without EGCG. The MTT assay was used to test the cytotoxicity of EGCG. Flow cytometry and DAPI staining were used to observe cell apoptosis caused by EGCG. To explore the effect of EGCG on the Indian Hedgehog signaling pathway and apoptosis-related proteins, RT-PCR and Western blotting were used to detect the expression of PTCH and Gli-1 in the Indian Hedgehog signaling pathway. Meanwhile, expression of Bcl-2, Bax, and caspase-3 were also evaluated by Western blot analysis.

Results EGCG effectively inhibited cellular proliferation and induced apoptosis of SW1353 and CRL-7891. EGCG inhibited the human Indian Hedgehog pathway, downregulated PTCH and Gli-1 levels, and induced apoptosis as confirmed by DAPI staining followed by flow cytometry. Protein expression levels of caspase-3 were unchanged in response to EGCG treatment in chondrosarcoma cells; however, the expression levels of Bcl-2 were significantly decreased and the levels of Bax were significantly increased.

Conclusions Our findings demonstrate that EGCG is effective for growth inhibition of a chondrosarcoma cell lines in vitro, and suggest that EGCG may be a new therapeutic option for patients with chondrosarcoma.

语种英语
所属项目编号RDN2007-15
资助者Peking University People&prime ; s Hospital Research and Development Funds ; Peking University People&prime ; s Hospital Research and Development Funds
WOS记录号WOS:000279824500008
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57863
专题北京大学第二临床医学院_骨肿瘤科
作者单位1.Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100044, Peoples R China
2.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100044, Peoples R China
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Tang, Guo-Qing,Yan, Tai-Qiang,Guo, Wei,et al. (-)-Epigallocatechin-3-gallate induces apoptosis and suppresses proliferation by inhibiting the human Indian Hedgehog pathway in human chondrosarcoma cells[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2010,136(8):1179-1185.
APA Tang, Guo-Qing.,Yan, Tai-Qiang.,Guo, Wei.,Ren, Ting-Ting.,Peng, Chang-Liang.,...&Han, Xiaoguang.(2010).(-)-Epigallocatechin-3-gallate induces apoptosis and suppresses proliferation by inhibiting the human Indian Hedgehog pathway in human chondrosarcoma cells.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,136(8),1179-1185.
MLA Tang, Guo-Qing,et al."(-)-Epigallocatechin-3-gallate induces apoptosis and suppresses proliferation by inhibiting the human Indian Hedgehog pathway in human chondrosarcoma cells".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 136.8(2010):1179-1185.
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