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学科主题临床医学
Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method
Li, Min1; Feng, Baosheng1; Liang, Yuan1; Zhang, Wei1; Bai, Yu1; Tang, Wen2; Wang, Tao2; Liu, Huwei1
关键词Lipidomics Free Fatty Acids Phospholipids Two-dimensional Liquid Chromatography-mass Spectrometry (2d Lc-ms) Potential Biomarkers Mia Syndrome
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2013-08-01
DOI10.1007/s00216-013-7109-5
405期:21页:6629-6638
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CHRONIC-RENAL-FAILURE ; MASS-SPECTROMETRY ; STRONG ASSOCIATION ; LIQUID ; FLUID
英文摘要

An improved online two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF MS) system was developed for the lipid profiling of human plasma, in which different lipid classes were separated by the first dimensional normal-phase (NP) LC and different lipid molecular species were separated by the second dimensional reversed-phase (RP) LC. This 2D LC-QToF MS system was built based on a ten-port, two-position valve as the interface, the conditions of which had been optimized and discussed in detail. As two loops were used to trap and transfer the first dimensional elute to the second dimension separately, this new interface suppressed the sample band broadening in the first dimensional column, increased the recovery and repeatability of 2D LC interface, and offered the possibility for the realization of not-stop-flow NP/RP 2D LC system. Finally, 190 endogenous lipid species out of 10 lipid classes were determined within a single run from the plasma of peritoneal patients. This method was also applied to identify the difference in lipid profile between plasma from peritoneal dialysis patients with bad volume status and peritoneal dialysis patients with good volume status. The discovery of 30 potential biomarkers would be helpful to the malnutrition, inflammation, and atherosclerosis syndrome investigation.

语种英语
所属项目编号21175005
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000322705600003
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57880
Collection北京大学第三临床医学院_肾内科
作者单位1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Inst Analyt Chem,Coll Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Div Nephrol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Li, Min,Feng, Baosheng,Liang, Yuan,et al. Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(21):6629-6638.
APA Li, Min.,Feng, Baosheng.,Liang, Yuan.,Zhang, Wei.,Bai, Yu.,...&Liu, Huwei.(2013).Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(21),6629-6638.
MLA Li, Min,et al."Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.21(2013):6629-6638.
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