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学科主题: 基础医学
题名:
Effect of kanglemycin C on lymphocyte proliferation induced by tetradecanoylphorbol acetate and ionomycin
作者: Li, JM; Lin, ZB
关键词: kanglemycin C ; cyclosporine ; tetradecanoylphorbol acetate ; ionomycin ; protein kinase C ; calcineurin ; lymphocytes ; cell division ; concanavalin A ; interleukin-2
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2000-02-01
卷: 21, 期:2, 页:188-192
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: CALCINEURIN PHOSPHATASE-ACTIVITY ; PROTEIN-KINASE-C ; CYCLOSPORINE-A ; T-LYMPHOCYTES ; EXPRESSION ; ACTIVATION ; BINDING
英文摘要:

AIM: To compare the suppressions of kanglemycin C (Kan) with that of cyclosporine (Cyc) on lymphocyte proliferations induced by tetradecanoylphorbol acetate (TPA) with ionomycin (IM), and concanavalin A (Con A). METHODS: Cell proliferation was quantified with 3-(4, 5-dimetyl-thiazol-2-yl)-2, 5-diphenyltetrazolium. bromide (MTT) or [H-3]thymidine ([H-3]TdR) incorporating method. Calcineurin (CN) activity was measured with molybdate dye colorimetry. RESULTS: Kan (8, 40, 80, and 400 nmol.L-1) strongly suppressed splenocyte proliferation induced by TPA and IM, and the suppressive effect of Kan gradually decreased along with the increasing concentrations of TPA (1 - 100 mu g.L-1) and IM (125 - 500 mu g.L-1). But, the suppression of Cyc on the splenocyte proliferation induced by TPA was mild. Cyc suppressed CN activity of mouse splenocytes stimulated by Con A stronger than Kan. Moreover, Kan and Cyc strongly suppressed spleen enriched T-cell proliferation induced by Con A and TPA + IM, and the suppression of Kan on proliferation was partly attenuated by exogenous IL-2. CONCLUSION: Kan competitively suppressed the proliferation induced by TPA and IM, and Cyc mainly suppressed IM part of the proliferation induced by TPA and IM.

语种: 英语
WOS记录号: WOS:000085208200016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57889
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: Beijing Med Univ, Dept Pharmacol, Beijing 100083, Peoples R China

Recommended Citation:
Li, JM,Lin, ZB. Effect of kanglemycin C on lymphocyte proliferation induced by tetradecanoylphorbol acetate and ionomycin[J]. ACTA PHARMACOLOGICA SINICA,2000,21(2):188-192.
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