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Production of transgenic mice expressing tumor virus A under ovarian-specific promoter 1 control using testis-mediated gene transfer
Chen, Xinhua1; Zhang, Zujuan1; Chang, Xiaohong1; Niu, Yidong2; Cui, Heng1
关键词Transgenic Mice Ovarian-specific Promoter Testis-mediated Gene Transfer
刊名MOLECULAR MEDICINE REPORTS
2014-03-01
DOI10.3892/mmr.2013.1876
9期:3页:955-960
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
资助者National Natural Science Foundation of China ; Peking University People&prime ; s Hospital ; National Natural Science Foundation of China ; Peking University People&prime ; s Hospital
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]IN-VIVO TRANSFECTION ; EPIDIDYMAL SPERM ; MOUSE MODELS ; GERM-CELLS ; RCAS-TVA ; DNA ; GENERATION ; CANCER ; ELECTROPORATION ; TRANSFORMATION
英文摘要

The aim of the present study was to produce transgenic mice expressing tumor virus A (TVA) in the ovary under ovarian specific promoter 1 (OSP1) control. A transgenic mouse model was established in which TVA, an avian retroviral receptor gene driven by OSP1, was selectively expressed in the ovary. A recombinant plasmid containing TVA cDNA and an OSP1 promoter was constructed. The DNA fragment was repeatedly injected into male mouse testes at multiple sites. At 4-7, 7-10 and 10-13 weeks following the final injection, two DNA-injected male mice were mated with four wild-type female mice to produce transgenic mice. The transgenic positive rate in mouse F1 offspring was 39.69%. When the positive F1 individuals were mated with wild-type Imprinting Control Region mice (PxW) or with positive F1 individuals (PxP), the F2 individuals had a transgenic rate of 12.44%. The transgenic rates in the F1 offspring, produced following mating at the three time intervals, were 55.71 (39/70), 30.77 (4/13) and 18.75% (9/48), respectively. The transgenic rates of the F2 offspring decreased with the age of the F1 offspring, from 26.67% when PxP were mated at 6-8 weeks of age to 6.52% when PxW were mated at 5-6 months of age. The results indicate a high efficiency of gene transfer to F1 offspring using testis-mediated gene transfer (TMGT). The transgenic rate in the F2 offspring was lower than that in the F1 offspring. The results reveal that TMGT is suitable for creating transgenic animals among F1 offspring. Semi-quantitative reverse transcription-polymerase chain reaction results showed that TVA was expressed in the mice ovaries. The results demonstrate the importance of using the replication-competent avian sarcoma-leukosis virus long terminal repeat with a splice acceptor-TVA system in ovarian tumorigenesis research.

语种英语
所属项目编号81172454 ; RDB2008-16
资助者National Natural Science Foundation of China ; Peking University People&prime ; s Hospital ; National Natural Science Foundation of China ; Peking University People&prime ; s Hospital
WOS记录号WOS:000332699000029
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57890
专题北京大学第二临床医学院_妇科肿瘤中心
作者单位1.Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Lab Anim Ctr, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Chen, Xinhua,Zhang, Zujuan,Chang, Xiaohong,et al. Production of transgenic mice expressing tumor virus A under ovarian-specific promoter 1 control using testis-mediated gene transfer[J]. MOLECULAR MEDICINE REPORTS,2014,9(3):955-960.
APA Chen, Xinhua,Zhang, Zujuan,Chang, Xiaohong,Niu, Yidong,&Cui, Heng.(2014).Production of transgenic mice expressing tumor virus A under ovarian-specific promoter 1 control using testis-mediated gene transfer.MOLECULAR MEDICINE REPORTS,9(3),955-960.
MLA Chen, Xinhua,et al."Production of transgenic mice expressing tumor virus A under ovarian-specific promoter 1 control using testis-mediated gene transfer".MOLECULAR MEDICINE REPORTS 9.3(2014):955-960.
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