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学科主题: 临床医学
题名:
Production of transgenic mice expressing tumor virus A under ovarian-specific promoter 1 control using testis-mediated gene transfer
作者: Chen, Xinhua1; Zhang, Zujuan1; Chang, Xiaohong1; Niu, Yidong2; Cui, Heng1
关键词: transgenic mice ; ovarian-specific promoter ; testis-mediated gene transfer
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2014-03-01
DOI: 10.3892/mmr.2013.1876
卷: 9, 期:3, 页:955-960
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: IN-VIVO TRANSFECTION ; EPIDIDYMAL SPERM ; MOUSE MODELS ; GERM-CELLS ; RCAS-TVA ; DNA ; GENERATION ; CANCER ; ELECTROPORATION ; TRANSFORMATION
英文摘要:

The aim of the present study was to produce transgenic mice expressing tumor virus A (TVA) in the ovary under ovarian specific promoter 1 (OSP1) control. A transgenic mouse model was established in which TVA, an avian retroviral receptor gene driven by OSP1, was selectively expressed in the ovary. A recombinant plasmid containing TVA cDNA and an OSP1 promoter was constructed. The DNA fragment was repeatedly injected into male mouse testes at multiple sites. At 4-7, 7-10 and 10-13 weeks following the final injection, two DNA-injected male mice were mated with four wild-type female mice to produce transgenic mice. The transgenic positive rate in mouse F1 offspring was 39.69%. When the positive F1 individuals were mated with wild-type Imprinting Control Region mice (PxW) or with positive F1 individuals (PxP), the F2 individuals had a transgenic rate of 12.44%. The transgenic rates in the F1 offspring, produced following mating at the three time intervals, were 55.71 (39/70), 30.77 (4/13) and 18.75% (9/48), respectively. The transgenic rates of the F2 offspring decreased with the age of the F1 offspring, from 26.67% when PxP were mated at 6-8 weeks of age to 6.52% when PxW were mated at 5-6 months of age. The results indicate a high efficiency of gene transfer to F1 offspring using testis-mediated gene transfer (TMGT). The transgenic rate in the F2 offspring was lower than that in the F1 offspring. The results reveal that TMGT is suitable for creating transgenic animals among F1 offspring. Semi-quantitative reverse transcription-polymerase chain reaction results showed that TVA was expressed in the mice ovaries. The results demonstrate the importance of using the replication-competent avian sarcoma-leukosis virus long terminal repeat with a splice acceptor-TVA system in ovarian tumorigenesis research.

语种: 英语
所属项目编号: 81172454 ; RDB2008-16
项目资助者: National Natural Science Foundation of China ; Peking University People&prime ; s Hospital
WOS记录号: WOS:000332699000029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57890
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Lab Anim Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Chen, Xinhua,Zhang, Zujuan,Chang, Xiaohong,et al. Production of transgenic mice expressing tumor virus A under ovarian-specific promoter 1 control using testis-mediated gene transfer[J]. MOLECULAR MEDICINE REPORTS,2014,9(3):955-960.
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