IR@PKUHSC  > 北京大学深圳医院  > 眼科
学科主题临床医学
Experimental study of TGF-beta 2 antisense oligodeoxynucleotide as an anti- scarring agent in glaucoma surgery
Li, Jin-Ying; Fu, Pei; Yang, Qi
关键词Tgf-beta 2 Antisense Therapy Glaucoma Surgery Scarring
刊名INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2008-12-18
1期:4页:315-319
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
资助者Technological Bureau of Shenzhen, China ; Technological Bureau of Shenzhen, China
研究领域[WOS]Ophthalmology
英文摘要

AIM: To investigate the effect of TGF-beta 2 antisense oligodeoxynucleotide on differentiation, proliferation of subconjunctival fibroblast following glaucoma filtration surgery.

METHODS: Glaucoma filtration surgery was performed on both eyes of 28 rabbits. TGF-beta 2 antisense oligodeoxynucleotide was subconjunctivally injected in the right eyes (group A),and TGF-beta 2 missense oligodeoxynucleotide (group B) or PBS (group C) was used at the same method in the left eyes as controls. Rabbits were killed at 4, 7, 14 and 28 days after surgery. Intraocular pressure (IOP), bleb characteristics were recorded at different time point. Subconjunctival fibroblasts were examined by immunohistochemistry and electron microscopy.

RESULTS: The TOP of rabbits in group A was significantly lower at 14 days (6.74 +/- 1.18mmHg) and 21 days (8.15 +/- 1.97mmHg) after operation than the TOP in group B (8.53 +/- 1.04, 9.72 +/- 1.09mmHg; P<0.01) and group C (8.79 +/- 1.21, 9.43 +/- 1.27mmHg; P<0.05). The mean bleb survival time was longer (17.2 days) in group A than that of group B (14.5 days) and group C (13.5 days) (P<0.05). The population of the cells expressing alpha -smooth muscle actin (alpha -SMA) and proliferating cell nuclear antigen (PCNA) was significantly reduced in group A compared with the group B and C. The ultrastructure of fibroblast was not altered by TGF-beta 2 antisense oligodeoxynucleotide.

CONCLUSION: TGF-beta 2 antisense oligodeoxynucleotide can prevent the scar formation after glaucoma surgery by inhibiting the differentiation and proliferation of subconjunctival fibroblast. It could be a potentially useful anti-scarring alternative for the prevention of late surgical failure.

语种英语
所属项目编号JH200505300483B
资助者Technological Bureau of Shenzhen, China ; Technological Bureau of Shenzhen, China
WOS记录号WOS:000207682600007
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57899
Collection北京大学深圳医院_眼科
作者单位Peking Univ, Shenzhen Hosp, Dept Ophthalmol, Shenzhen 518036, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Li, Jin-Ying,Fu, Pei,Yang, Qi. Experimental study of TGF-beta 2 antisense oligodeoxynucleotide as an anti- scarring agent in glaucoma surgery[J]. INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,2008,1(4):315-319.
APA Li, Jin-Ying,Fu, Pei,&Yang, Qi.(2008).Experimental study of TGF-beta 2 antisense oligodeoxynucleotide as an anti- scarring agent in glaucoma surgery.INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,1(4),315-319.
MLA Li, Jin-Ying,et al."Experimental study of TGF-beta 2 antisense oligodeoxynucleotide as an anti- scarring agent in glaucoma surgery".INTERNATIONAL JOURNAL OF OPHTHALMOLOGY 1.4(2008):315-319.
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