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学科主题: 临床医学
题名:
Experimental study of TGF-beta 2 antisense oligodeoxynucleotide as an anti- scarring agent in glaucoma surgery
作者: Li, Jin-Ying; Fu, Pei; Yang, Qi
关键词: TGF-beta 2 ; antisense therapy ; glaucoma surgery ; scarring
刊名: INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
发表日期: 2008-12-18
卷: 1, 期:4, 页:315-319
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
英文摘要:

AIM: To investigate the effect of TGF-beta 2 antisense oligodeoxynucleotide on differentiation, proliferation of subconjunctival fibroblast following glaucoma filtration surgery.

METHODS: Glaucoma filtration surgery was performed on both eyes of 28 rabbits. TGF-beta 2 antisense oligodeoxynucleotide was subconjunctivally injected in the right eyes (group A),and TGF-beta 2 missense oligodeoxynucleotide (group B) or PBS (group C) was used at the same method in the left eyes as controls. Rabbits were killed at 4, 7, 14 and 28 days after surgery. Intraocular pressure (IOP), bleb characteristics were recorded at different time point. Subconjunctival fibroblasts were examined by immunohistochemistry and electron microscopy.

RESULTS: The TOP of rabbits in group A was significantly lower at 14 days (6.74 +/- 1.18mmHg) and 21 days (8.15 +/- 1.97mmHg) after operation than the TOP in group B (8.53 +/- 1.04, 9.72 +/- 1.09mmHg; P<0.01) and group C (8.79 +/- 1.21, 9.43 +/- 1.27mmHg; P<0.05). The mean bleb survival time was longer (17.2 days) in group A than that of group B (14.5 days) and group C (13.5 days) (P<0.05). The population of the cells expressing alpha -smooth muscle actin (alpha -SMA) and proliferating cell nuclear antigen (PCNA) was significantly reduced in group A compared with the group B and C. The ultrastructure of fibroblast was not altered by TGF-beta 2 antisense oligodeoxynucleotide.

CONCLUSION: TGF-beta 2 antisense oligodeoxynucleotide can prevent the scar formation after glaucoma surgery by inhibiting the differentiation and proliferation of subconjunctival fibroblast. It could be a potentially useful anti-scarring alternative for the prevention of late surgical failure.

语种: 英语
所属项目编号: JH200505300483B
项目资助者: Technological Bureau of Shenzhen, China
WOS记录号: WOS:000207682600007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57899
Appears in Collections:北京大学深圳医院_眼科_期刊论文

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作者单位: Peking Univ, Shenzhen Hosp, Dept Ophthalmol, Shenzhen 518036, Guangdong, Peoples R China

Recommended Citation:
Li, Jin-Ying,Fu, Pei,Yang, Qi. Experimental study of TGF-beta 2 antisense oligodeoxynucleotide as an anti- scarring agent in glaucoma surgery[J]. INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,2008,1(4):315-319.
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