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学科主题基础医学
TMEM74, a lysosome and autophagosome protein, regulates autophagy
Yu, Chuanfei2,3; Wang, Lan2,3; Lv, Bingfeng2,3; Lu, Yang1; Zeng, Ling′ e(2,3); Chen, Yingyu2,3; Ma, Dalong1,2,3; Shi, Taiping1,2,3; Wang, Lu2,3
关键词autophagy lysosome autophagosome TMEM74
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2008-05-02
DOI10.1016/j.bbrc.2008.02.055
369期:2页:622-629
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]CELL ; DEGRADATION ; VACUOLES ; YEAST ; APOPTOSIS ; MEMBRANES
英文摘要

Autophagy is an intracellular degradation/recycling process in eukaryotic cells. It contributes to the turnover of cellular components by delivering portions of the cytoplasm and organelles to lysosomes for digestion. The molecular mechanisms of autophagy and vesicle trafficking, especially the biogenesis and turnover of autophagosomes, are poorly understood. In this report, we describe the biological activity of a novel autophagy-related molecule, FLJ30668, or Transmembrane protein 74 (TMEM74). Its transcript was identified by Northern blot and the open reading frame was found to encode 393 amino acids, which shared very little identity with other genetic products. Subcellular localization analysis showed TMEM74 localized to the lysosome and autophagosome. Overexpression of TMEM74 in HeLa cells resulted in autophagic vacuolization, increased the dotted distribution of MDC and GFP-LC3, and endogenous LC3-II levels. Wortmannin, an autophagy inhibitor, partially attenuated these effects. Moreover, knockdown of TMEM74 by small interference RNA abolished the autophagic characteristics induced by starvation. These findings demonstrate that TMEM74 may be involved in promoting functional autophagy during cell starvation and other stress conditions. (c) 2008 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000254676900056
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被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/57905
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
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Yu, Chuanfei,Wang, Lan,Lv, Bingfeng,et al. TMEM74, a lysosome and autophagosome protein, regulates autophagy[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,369(2):622-629.
APA Yu, Chuanfei.,Wang, Lan.,Lv, Bingfeng.,Lu, Yang.,Zeng, Ling&prime.,...&Wang, Lu.(2008).TMEM74, a lysosome and autophagosome protein, regulates autophagy.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,369(2),622-629.
MLA Yu, Chuanfei,et al."TMEM74, a lysosome and autophagosome protein, regulates autophagy".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 369.2(2008):622-629.
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