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学科主题: 基础医学
题名:
Immunolocalization of steroidogenic enzymes and their expression during the breeding season in the testes of wild raccoon dogs (Nyctereutes procyonoides)
作者: Weng, Qiang1,4; Tsubota, Toshio3; Dai, Mingdao; Weng, Jiaju2; Tian, Yang; Xu, Meiyu; Watanabe, Gen4,5; Taya, Kazuyoshi4,5
关键词: immunohistochemistry ; mRNA ; raccoon dog ; steroidogenic enzymes ; testes
刊名: ANIMAL SCIENCE JOURNAL
发表日期: 2012
DOI: 10.1111/j.1740-0929.2011.00990.x
卷: 83, 期:7, 页:535-542
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Agriculture, Dairy & Animal Science
研究领域[WOS]: Agriculture
关键词[WOS]: URSUS-THIBETANUS-JAPONICUS ; CITELLUS-DAURICUS-BRANDT ; INHIBIN/ACTIVIN SUBUNITS ; MELATONIN IMPLANTS ; SPERMATOGENESIS ; LOCALIZATION ; SPERMATIDS ; AROMATASE
英文摘要:

The objective of this study was to investigate immunolocalization of steroidogenic enzymes 3 beta HSD, P450c17 and P450arom and their expression during the breeding season in wild male raccoon dogs. The testicular weight, size and seminiferous tubule diameters were measured, and histological and immunohistochemical observations of testes were performed. The messenger RNA expression (mRNA) of 3 beta HSD, P450c17 and P450arom was measured in the testes during the breeding season. 3 beta HSD was found in Leydig cells during the breeding and non-breeding seasons with more intense staining in the breeding season. P450c17 was identified in Leydig cells and spermatids in the breeding season, whereas it was present only in Leydig cells in the non-breeding season. The localization of P450arom changed seasonally: no immunostaining in the non-breeding season; more extensive immunostaining in Leydig cells, Sertoli cells and elongating spermatids in the breeding season. In addition, 3 beta HSD, P450c17 and P450arom mRNA were also expressed in the testes during the breeding season. These results suggested that seasonal changes in testicular weight, size and seminiferous tubule diameter in the wild raccoon dog were correlated with spermatogenesis and immunoreactivity of steroidogenic enzymes and that steroidogenic enzymes may play an important role in the spermatogenesis and testicular recrudescence and regression process.

语种: 英语
所属项目编号: IRT0607
项目资助者: Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of China ; Program for Changjiang Scholars and Innovative Research Team in Universities from China
WOS记录号: WOS:000306220200003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57908
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Beijing 100871, Peoples R China
2.Gifu Univ, United Grad Sch Vet Sci, Gifu, Japan
3.Beijing Forestry Univ, Coll Biol Sci & Technol, Physiol Anim Lab, Beijing 100083, Peoples R China
4.Hokkaido Univ, Grad Sch Vet Med, Lab Wildlife Biol, Kita Ku, Sapporo, Hokkaido, Japan
5.Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Med, Lab Vet Physiol, Fuchu, Tokyo 183, Japan

Recommended Citation:
Weng, Qiang,Tsubota, Toshio,Dai, Mingdao,et al. Immunolocalization of steroidogenic enzymes and their expression during the breeding season in the testes of wild raccoon dogs (Nyctereutes procyonoides)[J]. ANIMAL SCIENCE JOURNAL,2012,83(7):535-542.
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