IR@PKUHSC  > 北京大学第三临床医学院  > 神经内科
学科主题临床医学
Adenosine monophosphate-activated protein kinase activation enhances embryonic neural stem cell apoptosis in a mouse model of amyotrophic lateral sclerosis
Sui, Yanling; Zhao, Zichun; Liu, Rong; Cal, Bin; Fan, Dongsheng
关键词Nerve Regeneration Neuroderegeneration Embryonic Neural Stem Cells Adenosine Monophosphate-activated Protein Kinase Alpha Paired Box 3 P53 Sod1(G93a) Mouse Amyotrophic Lateral Sclerosis Oxidative Stress Hydrogen Peroxide Apoptosis Nsfc Grants Neural Regeneration
刊名NEURAL REGENERATION RESEARCH
2014-10-01
DOI10.4103/1673-5374.143421
9期:19页:1770-1778
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Neurosciences
研究领域[WOS]Cell Biology ; Neurosciences & Neurology
关键词[WOS]2-HIT HYPOTHESIS ; PROGENITOR CELLS ; DISEASE ; ENERGY ; MICE ; PROLIFERATION ; DEGENERATION ; NEUROGENESIS ; METABOLISM ; MUTANT
英文摘要

Alterations in embryonic neural stem cells play crucial roles in the pathogenesis of amyotrophic lateral sclerosis. We hypothesized that embryonic neural stem cells from SOD1(G93A) individuals might be more susceptible to oxidative injury, resulting in a propensity for neurodegeneration at later stages. In this study, embryonic neural stem cells obtained from human superoxide dismutase 1 mutant (SOD1(G93A)) and wild-type (SOD1(WT)) mouse models were exposed to H2O2. We assayed cell viability with mitochondrial succinic dehydrogenase colorimetric reagent, and measured cell apoptosis by flow cytometry. Moreover, we evaluated the expression of the adenosine monophosphate-activated protein kinase (AMPK) alpha-subunit, paired box 3 (Pax3) protein, and p53 in western blot analyses. Compared with SOD1(WT) cells, SOD1(G93A) embryonic neural stem cells were more likely to undergo H2O2-induced apoptosis. Phosphorylation of AMPK alpha in SOD1(G93A) cells was higher than that in SOD1(WT) cells. Pax3 expression was inversely correlated with the phosphorylation levels of AMPK alpha. p53 protein levels were also correlated with AMPKa phosphorylation levels. Compound C, an inhibitor of AMPK alpha, attenuated the effects of H2O2. These results suggest that embryonic neural stem cells from SOD1(G93A) mice are more susceptible to apoptosis in the presence of oxidative stress compared with those from wild-type controls, and the effects are mainly mediated by Pax3 and p53 in the AMPK alpha pathway.

语种英语
WOS记录号WOS:000344637100010
项目编号81030019
资助机构National Natural Sciences Foundation of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57920
专题北京大学第三临床医学院_神经内科
作者单位Peking Univ, Hosp 3, Dept Neurol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Sui, Yanling,Zhao, Zichun,Liu, Rong,et al. Adenosine monophosphate-activated protein kinase activation enhances embryonic neural stem cell apoptosis in a mouse model of amyotrophic lateral sclerosis[J]. NEURAL REGENERATION RESEARCH,2014,9(19):1770-1778.
APA Sui, Yanling,Zhao, Zichun,Liu, Rong,Cal, Bin,&Fan, Dongsheng.(2014).Adenosine monophosphate-activated protein kinase activation enhances embryonic neural stem cell apoptosis in a mouse model of amyotrophic lateral sclerosis.NEURAL REGENERATION RESEARCH,9(19),1770-1778.
MLA Sui, Yanling,et al."Adenosine monophosphate-activated protein kinase activation enhances embryonic neural stem cell apoptosis in a mouse model of amyotrophic lateral sclerosis".NEURAL REGENERATION RESEARCH 9.19(2014):1770-1778.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sui, Yanling]的文章
[Zhao, Zichun]的文章
[Liu, Rong]的文章
百度学术
百度学术中相似的文章
[Sui, Yanling]的文章
[Zhao, Zichun]的文章
[Liu, Rong]的文章
必应学术
必应学术中相似的文章
[Sui, Yanling]的文章
[Zhao, Zichun]的文章
[Liu, Rong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。