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Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.260 from Streptococcus mutans - a cariogenic dental pathogen
Zhang, XY; Mi, W; Zhou, YF; Liu, XY; Li, LF; Liang, YH; Wei, SC; Su, XD
关键词Streptococcus Mutans Dental Caries Smu.260 Protein Crystallography
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2005-03-01
32期:3页:217-220
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]SEQUENCE
英文摘要

Smu.260 encodes a putative protein of 200 residues in Streptococcus mutans, a primary pathogen for human dental caries. Smu.260 was cloned into expression vector pET28a and expressed in good amount from the E.coli strain BL21 (DE3). Smu.260 protein was purified to homogeneity in a two-step procedure of Ni(2+) chelating and size exclusion chromatography. The purified protein exists in two forms, a dimer form about 46 ku with yellow color and a tetramer form without apparent color. Crystals were obtained from the dimer protein by hanging-drop vapor-diffusion method. The crystals diffracted to about 2.3 angstrom resolution and belong to orthorhombic space group P2(1)2(1)2(1) with cell dimensions of a = 89.88 angstrom, b = 90.91 angstrom, c = 105.17 angstrom. The asymmetric unit is expected to contain two dimers with solvent content of 53%.

语种中文
WOS记录号WOS:000227789000005
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57923
专题北京大学口腔医学院
作者单位1.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
3.Peking Univ, Coll Life Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
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GB/T 7714
Zhang, XY,Mi, W,Zhou, YF,et al. Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.260 from Streptococcus mutans - a cariogenic dental pathogen[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(3):217-220.
APA Zhang, XY.,Mi, W.,Zhou, YF.,Liu, XY.,Li, LF.,...&Su, XD.(2005).Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.260 from Streptococcus mutans - a cariogenic dental pathogen.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(3),217-220.
MLA Zhang, XY,et al."Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.260 from Streptococcus mutans - a cariogenic dental pathogen".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.3(2005):217-220.
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