北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 期刊论文
学科主题: 口腔医学
题名:
Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.260 from Streptococcus mutans - a cariogenic dental pathogen
作者: Zhang, XY; Mi, W; Zhou, YF; Liu, XY; Li, LF; Liang, YH; Wei, SC; Su, XD
关键词: Streptococcus mutans ; dental caries ; Smu.260 ; protein crystallography
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2005-03-01
卷: 32, 期:3, 页:217-220
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: SEQUENCE
英文摘要:

Smu.260 encodes a putative protein of 200 residues in Streptococcus mutans, a primary pathogen for human dental caries. Smu.260 was cloned into expression vector pET28a and expressed in good amount from the E.coli strain BL21 (DE3). Smu.260 protein was purified to homogeneity in a two-step procedure of Ni(2+) chelating and size exclusion chromatography. The purified protein exists in two forms, a dimer form about 46 ku with yellow color and a tetramer form without apparent color. Crystals were obtained from the dimer protein by hanging-drop vapor-diffusion method. The crystals diffracted to about 2.3 angstrom resolution and belong to orthorhombic space group P2(1)2(1)2(1) with cell dimensions of a = 89.88 angstrom, b = 90.91 angstrom, c = 105.17 angstrom. The asymmetric unit is expected to contain two dimers with solvent content of 53%.

语种: 中文
WOS记录号: WOS:000227789000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57923
Appears in Collections:北京大学口腔医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
3.Peking Univ, Coll Life Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, XY,Mi, W,Zhou, YF,et al. Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.260 from Streptococcus mutans - a cariogenic dental pathogen[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(3):217-220.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, XY]'s Articles
[Mi, W]'s Articles
[Zhou, YF]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, XY]‘s Articles
[Mi, W]‘s Articles
[Zhou, YF]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace