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IR@PKUHSC  > 北京大学第一临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Protective effects of intermedin/adrenomedullin2 on ischemia/reperfusion injury in isolated rat hearts
作者: Yang, JH; Qi, YF; Jia, YX; Pan, CS; Zhao, J; Yang, J; Chang, JK; Tang, CS
关键词: intermedin ; ischemia/reperfusion ; cAMP ; heart
刊名: PEPTIDES
发表日期: 2005-03-01
DOI: 10.1016/j.peptides.2004.10.025
卷: 26, 期:3, 页:501-507
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: GENE-RELATED PEPTIDE ; ACTIVITY-MODIFYING PROTEINS ; ADRENOMEDULLIN ; IDENTIFICATION ; EXPRESSION ; RECEPTORS ; AMYLIN ; FAMILY
英文摘要:

Intermedin (IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CT/CGRP) family identified from human and other vertebrate tissues. Preprointermedin can generate a 47-arnino acid mature peptide (IMD1-47) and a shorter 40-amino acid one (IMD8-47) by proteolytic cleavage. The present study was designed to determine the protective effect of IMD on cardiac ischemia/reperfusion (I/R) injury and its possible mechanism. Isolated rat hearts were perfused on a Langendorff apparatus and subjected to 45-min global ischemia and 30-min reperfusion. Cardiac function was measured. The release of myocardial protein and lactate dehydrogenase (LDH) and the formation of malondialdehyde (MDA) were assayed. Myocardial cAMP content was determined by radioimmunoassay (RIA). Cardiac I/R induced a marked inhibition of cardiac function and myocardial injury. Reperfusion with IMD significantly attenuated the I/R injury. Compared with I/R alone. perfusion with 10(-8) mol/L IMD1-47 and IMD8-47 induced a 36% and 33% increase in Delta left ventricular pressure (DeltaLVP), 30% and 28% in maximal rate of increase of LV pressure (+LVdP/dt max), and 34% and 31 % in maximal rate of decrease of LV pressure (-LVdP/dt max). respectively (all P<0.01) but an approximately 58% and 51% decrease in LV diastolic pressure, respectively (P<0.01). In addition, perfusion with IMD markedly attenuated the leakage of LDH, total protein and myoglobin from myocardia compared with I/R alone. The contents of ventricular myocardia cAMP after reperfusion with 10(-8) mol/L IMD1-47 and IMD8-47 were 130% and 91% higher, respectively, than that with I/R alone (all P<0.01). However, formations of myocardial MDA were 52% and 50% lower than that with I/R alone (all P<0.01), respectively. Interestingly, the above IMD effects were similar to those of adrenomedullin (10-8 mol/L). These results suggest that IMD. like adrenomedullin. exerts cardio-protective effects against myocardial I/R injury. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000226932900018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57928
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ First Hosp, Inst Cardiovasc Res, Beijing 100034, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
3.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
4.Phoenix Pharmaceut Inc, Belmont, CA 94002 USA

Recommended Citation:
Yang, JH,Qi, YF,Jia, YX,et al. Protective effects of intermedin/adrenomedullin2 on ischemia/reperfusion injury in isolated rat hearts[J]. PEPTIDES,2005,26(3):501-507.
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