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Targeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes
Quan, Guangnan1; Du, Xiangke1; Huo, Tianlong1; Li, Xubin1; Wei, Zhengmao1; Cui, Heng2; Chang, Xiaohong2; Cheng, Yexia2; Ye, Xue2; Cheng, Hongyan2
关键词Ovarian Cancer Oc183b2 Magnetic Resonance Imaging Ultrasmall Superparamagnetic Iron Oxide Molecular Imaging
刊名ACADEMIC RADIOLOGY
2010-12-01
DOI10.1016/j.acra.2010.07.006
17期:12页:1468-1476
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]ONE-POT REACTION ; MAGNETITE NANOPARTICLES ; MONOCLONAL-ANTIBODY ; CONTRAST AGENT ; IRON-OXIDE ; CARCINOMA ; TUMORS ; NANOCRYSTALS
英文摘要

Rationale and Objectives: This study was designed to develop a novel magnetic resonance (MR) probe for the antigen OC183B2 in ovarian cancer cells and investigate its imaging features in vitro and in vivo.

Materials and Methods: Molecular probes were achieved through ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) conjugated to ovarian cancer monoclonal antibodies 183B2 (OCMab183B2) using a chemical method. In the control group, USPIOs were coupled with murine immunoglobulin G (mIgG) and conjugated the same way. Native polyacrylamide gel electrophoresis was used to evaluate the conjugation reaction. The cytotoxicity of the probe was measured using the methyl thiazolyl tetrazolium assay, and its cell-labeling efficiency was evaluated by Prussian blue staining. In vitro cell MR imaging was performed to evaluate the targeting of the probe to the cells. After that, the OCMab183B2 USPIOs and mIgG USPIOs were injected intravenously into nude mice implanted with ovarian cancer xenograft tumors, respectively. T2-weighted imaging and T2 mapping were then performed on a 3.0-T MR imaging system equipped with an animal birdcage coil at different times. Finally, the nude mice were sacrificed for histologic examination to confirm the imaging results.

Results: Native polyacrylamide gel electrophoresis displayed an optimal conjugation of USPIOs to OCMab183B2 and mIgG. Various blue-staining particles were found in the cells labeled with the molecular probe at different iron concentrations, and the density of particles was positively related to the iron concentration. Its labeling rate was 96.06%, which was higher than that of USPIOs (62.5%) at the same iron concentration (20 mu g/mL). The methyl thiazolyl tetrazolium assay showed that there was no difference in cellular bioactivity between OCMab183B2 USPIO-labeled and nonlabeled cells (P>.05). In vitro cell MR imaging showed that there was an obvious decrease in signal intensity for the probe-labeled cells compared to mIgG USPIO-labeled cells. For in vivo MR imaging, distinct changes of signal intensities and T2 values of ovarian cancers were detected after the injection of OCMab183B2 USPIOs compared to mIgG USPIOs. The histologic analysis showed that iron depositions were visualized in the experimental group but not in the control group.

Conclusion: OCMab183B2 USPIO conjugates have the potential to be useful as OC183B2-targeted MR imaging agents for the early detection of ovarian cancers.

语种英语
WOS记录号WOS:000284553900003
项目编号7092111
资助机构Beijing Nature Science Foundation (Beijing, China)
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57943
专题北京大学第二临床医学院_妇科肿瘤中心
北京大学第二临床医学院_妇科
北京大学第二临床医学院_放射科
作者单位1.Peking Univ, Peoples Hosp, Dept Radiol, Beijing 100871, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gynecol Oncol Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Quan, Guangnan,Du, Xiangke,Huo, Tianlong,et al. Targeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes[J]. ACADEMIC RADIOLOGY,2010,17(12):1468-1476.
APA Quan, Guangnan.,Du, Xiangke.,Huo, Tianlong.,Li, Xubin.,Wei, Zhengmao.,...&Cheng, Hongyan.(2010).Targeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes.ACADEMIC RADIOLOGY,17(12),1468-1476.
MLA Quan, Guangnan,et al."Targeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes".ACADEMIC RADIOLOGY 17.12(2010):1468-1476.
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