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学科主题: 临床医学
题名:
Targeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes
作者: Quan, Guangnan1; Du, Xiangke1; Huo, Tianlong1; Li, Xubin1; Wei, Zhengmao1; Cui, Heng2; Chang, Xiaohong2; Cheng, Yexia2; Ye, Xue2; Cheng, Hongyan2
关键词: Ovarian cancer ; OC183B2 ; magnetic resonance imaging ; ultrasmall superparamagnetic iron oxide ; molecular imaging
刊名: ACADEMIC RADIOLOGY
发表日期: 2010-12-01
DOI: 10.1016/j.acra.2010.07.006
卷: 17, 期:12, 页:1468-1476
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: ONE-POT REACTION ; MAGNETITE NANOPARTICLES ; MONOCLONAL-ANTIBODY ; CONTRAST AGENT ; IRON-OXIDE ; CARCINOMA ; TUMORS ; NANOCRYSTALS
英文摘要:

Rationale and Objectives: This study was designed to develop a novel magnetic resonance (MR) probe for the antigen OC183B2 in ovarian cancer cells and investigate its imaging features in vitro and in vivo.

Materials and Methods: Molecular probes were achieved through ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) conjugated to ovarian cancer monoclonal antibodies 183B2 (OCMab183B2) using a chemical method. In the control group, USPIOs were coupled with murine immunoglobulin G (mIgG) and conjugated the same way. Native polyacrylamide gel electrophoresis was used to evaluate the conjugation reaction. The cytotoxicity of the probe was measured using the methyl thiazolyl tetrazolium assay, and its cell-labeling efficiency was evaluated by Prussian blue staining. In vitro cell MR imaging was performed to evaluate the targeting of the probe to the cells. After that, the OCMab183B2 USPIOs and mIgG USPIOs were injected intravenously into nude mice implanted with ovarian cancer xenograft tumors, respectively. T2-weighted imaging and T2 mapping were then performed on a 3.0-T MR imaging system equipped with an animal birdcage coil at different times. Finally, the nude mice were sacrificed for histologic examination to confirm the imaging results.

Results: Native polyacrylamide gel electrophoresis displayed an optimal conjugation of USPIOs to OCMab183B2 and mIgG. Various blue-staining particles were found in the cells labeled with the molecular probe at different iron concentrations, and the density of particles was positively related to the iron concentration. Its labeling rate was 96.06%, which was higher than that of USPIOs (62.5%) at the same iron concentration (20 mu g/mL). The methyl thiazolyl tetrazolium assay showed that there was no difference in cellular bioactivity between OCMab183B2 USPIO-labeled and nonlabeled cells (P>.05). In vitro cell MR imaging showed that there was an obvious decrease in signal intensity for the probe-labeled cells compared to mIgG USPIO-labeled cells. For in vivo MR imaging, distinct changes of signal intensities and T2 values of ovarian cancers were detected after the injection of OCMab183B2 USPIOs compared to mIgG USPIOs. The histologic analysis showed that iron depositions were visualized in the experimental group but not in the control group.

Conclusion: OCMab183B2 USPIO conjugates have the potential to be useful as OC183B2-targeted MR imaging agents for the early detection of ovarian cancers.

语种: 英语
所属项目编号: 7092111
项目资助者: Beijing Nature Science Foundation (Beijing, China)
WOS记录号: WOS:000284553900003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57943
Appears in Collections:北京大学第二临床医学院_放射科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Radiol, Beijing 100871, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gynecol Oncol Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Quan, Guangnan,Du, Xiangke,Huo, Tianlong,et al. Targeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes[J]. ACADEMIC RADIOLOGY,2010,17(12):1468-1476.
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