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学科主题: 临床医学
题名:
Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis
作者: Long, Li1,2,3; Yu, Ping1,4; Liu, Yanying1; Wang, Shiyao1; Li, Ru1; Shi, Jinxia1; Zhang, Xiaoping1,5; Li, Yanmei1,5; Sun, Xiaolin1; Zhou, Bin2,3; Cui, Liufu4; Li, Zhanguo1
刊名: CLINICAL & DEVELOPMENTAL IMMUNOLOGY
发表日期: 2013
DOI: 10.1155/2013/296139
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: BIOGENESIS ; COMPLEX ; ROLES
英文摘要:

Objective. This study was to screen for the miRNAs differently expressed in peripheral blood mononuclear cells (PBMC) of RA, to further identify the expression of miR-155 in RA PBMC and fibroblast-like synoviocytes (FLS), and to evaluate the function of miR-155 in RA-FLS. Methods. Microarray was used to screen for differentially expressed miRNAs in RA PBMC. miR-155 expression in PBMC and FLS of RA were identified by real-time PCR. Enforced overexpression and downexpression of miR-155 were used to investigate the function of miR-155 in RA-FLS. Expression of IKBKE which was previously identified as the actual target of miR-155 was examined by Western blot and real-time PCR in RA-FLS. Results. miR-155 levels were increased in both PBMC and FLS of RA and could be induced by TNF-alpha. Upregulation of miR-155 decreased MMP-3 levels and suppressed proliferation and invasion of RA-FLS. Inverse relationship between the expressions of miR-155 and the MMPs production-related protein IKBKE was found. Conclusion. An inflammatory milieu may alter miRNA expression profiles in rheumatoid arthritis. miR-155 is upregulated in RA-FLS, and it may be a protective factor against the inflammatory effect in part by attenuating expression of IKBKE.

语种: 英语
所属项目编号: 2008BA159B01 ; 81102272 ; 12040820382 ; 2010CB529100
项目资助者: National Key Technology R&amp ; D Program in the 11th Five Year Plan of China ; National Natural Science Foundation of China ; Chinese Medical Association Foundation ; Major State Basic Research Development Program of China (973 Program)
WOS记录号: WOS:000325064700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57949
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Sichuan Prov Peoples Hosp, Chengdu 610072, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China
3.Sichuan Acad Med Sci, Dept Rheumatol & Immunol, Chengdu 610072, Peoples R China
4.Kailuan Gen Hosp, Dept Rheumatol & Immunol, Tangshan 063000, Peoples R China
5.Weifang Med Univ, Dept Rheumatol & Immunol, Weifang 261053, Peoples R China

Recommended Citation:
Long, Li,Yu, Ping,Liu, Yanying,et al. Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis[J]. CLINICAL & DEVELOPMENTAL IMMUNOLOGY,2013.
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