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学科主题: 临床医学
题名:
Graft-versus-leukemia effects of Wilms′ tumor 1 protein-specific cytotoxic T lymphocytes in patients with chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation
作者: Wang Zhi-dong; Li Dan; Huang Xiao-jun
关键词: cytotoxic T cells ; Wilms&prime ; tumor 1 protein ; graft-versus-leukemia effects ; chronic myeloid leukemia ; allogeneic hematopoietic stem cell transplantation
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-04-05
DOI: 10.3760/cma.j.issn.0366-6999.2010.07.027
卷: 123, 期:7, 页:912-916
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: RESIDUAL DISEASE ; RESPONSES ; MEMORY ; WT1 ; EXPRESSION ; PRODUCT ; TRIAL ; CD57
英文摘要:

Background The role of Wilms′ tumor 1 protein (WT1)-specific cytotoxic T cells (CTL) in eradicating chronic myeloid leukemia (CML) cells is to be established. The aim of this study was to determine whether WT1 contributed to the graft-versus-leukemia effects (GVLE) for CML following allogeneic hematopoietic stem cell transplantation (HSCT).

Methods High-resolution human leukocyte antigen (HLA) class I genotyping was performed by sequence-specific polymerase chain reaction (PCR). Fifteen HLA-A*2402 patients with CML who underwent allogeneic HSCT were enrolled in this study. We monitored the frequency of WT1-specific CTL by pentamer assay and the molecular minimal residual disease by real-time quantitative PCR.

Results A CD8(+) T-cell response to WT1 was observed in 14 of 15 patients after HSCT. The median frequencies of WT1-CTL were 0.54%, 0.62%, 0.81% and 1.28% (%CD8) on days 30, 60, 90 and 180, respectively. The median frequency of WT1-CTL (1.38%) in patients with molecular remission (MoR) was significantly higher than that in those without MoR (0.38%) on day 30, while no significant differences between them were detected on days 60, 90 and 180. The increase of WT1-CTL was associated with a decrease in bcr-abl expression and MoR; and the decrease of WT1-CTL was associated with an increase in bcr-abl expression, suggesting a WT1-driven GVL effect. WT1-CTL had a predominant effector-memory phenotype (CD45RO(+)CD27(-)CD57(+)).

Conclusions The emergence of WT1-CTL with an effector-memory phenotype is associated with GVLE in CML patients after HSCT. This will pave the way for the WT1 vaccines to enhance GVLE after HSCT in CML. Chin Med J 2010;123(7):912-916

语种: 英语
所属项目编号: 30725038 ; IRT0702 ; 2006AA02Z4A0 ; 20060001108
项目资助者: Natural Science Foundation ; National 863 Hi-tech Project ; Invocation Team Fund ; Ministry of Education of China
WOS记录号: WOS:000276683700027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57956
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China

Recommended Citation:
Wang Zhi-dong,Li Dan,Huang Xiao-jun. Graft-versus-leukemia effects of Wilms′ tumor 1 protein-specific cytotoxic T lymphocytes in patients with chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation[J]. CHINESE MEDICAL JOURNAL,2010,123(7):912-916.
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