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学科主题: 基础医学
题名:
Effects of Nt-truncation and coexpression of isolated Nt domains on the membrane trafficking of electroneutral Na+/HCO3- cotransporters
作者: Wang, Deng-Ke1; Liu, Ying1; Myers, Evan J.2; Guo, Yi-Min1; Xie, Zhang-Dong1; Jiang, De-Zhi1; Li, Jia-Min1; Yang, Jichun3; Liu, Mugen4; Parker, Mark D.2; Chen, Li-Ming1
刊名: SCIENTIFIC REPORTS
发表日期: 2015-07-20
DOI: 10.1038/srep12241
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: BIMOLECULAR FLUORESCENCE COMPLEMENTATION ; COUPLED BICARBONATE TRANSPORTERS ; SLC4A10 SPLICE VARIANTS ; NA/HCO3 COTRANSPORTER ; FUNCTIONAL-CHARACTERIZATION ; TISSUE DISTRIBUTION ; CL-/HCO3-EXCHANGER ; ANION-EXCHANGER ; NBCN1 SLC4A7 ; MOUSE-BRAIN
英文摘要:

The SLC4 genes are all capable of producing multiple variants by alternative splicing or using alternative promoters. The physiological consequences of such diversity are of great interest to investigators. Here, we identified two novel variants of the electroneutral Na+/HCO3- cotransporter NBCn1, one full-length starting with "MIPL" and the other Nt-truncated starting with "MDEL". Moreover, we identified a new promoter of Slc4a10 encoding NBCn2 and a novel type of Nt-truncated NBCn2 starting with "MHAN". When heterologously expressed, the new NBCn1 variants were well localized to the plasma membrane and exhibited characteristic NBCn1 activity. However, MHAN-NBCn2 was poorly localized on the plasma membrane. By deletion mutations, we identified NBCn2 greatly enhances the surface abundance of the Nt-truncated NBCn2. Coimmunoprecipitation and bimolecular fluorescence complementation studies showed that the fulllength and Nt-truncated NBCn2 interact with each other to form heterodimers in neuro-2A cells. Finally, we showed that the isolated Nt domain interacts with and enhances the surface abundance of the Nt-truncated NBCn2. The present study expands our knowledge of the NBCn1 and NBCn2 transcriptome, and provides insights into how the Nt domain could affect transporter function by regulating its membrane trafficking.

语种: 英语
所属项目编号: 31371171 ; 30900513 ; 2013TS083 ; 2014TS086
项目资助者: Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities of China ; School of Medicine and Biomedical Sciences ; Department of Physiology and Biophysics at UB:SUNY
WOS记录号: WOS:000358148200001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57957
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.SUNY Buffalo, Sch Med, Dept Physiol & Biophys, Buffalo, NY 14214 USA
2.Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Dept Biophys & Mol Physiol, Key Lab Mol Biophys,Minist Educ, Wuhan 430074, Hubei, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100091, Peoples R China
4.Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Dept Genet & Dev Biol, Key Lab Mol Biophys,Minist Educ, Wuhan 430074, Hubei, Peoples R China

Recommended Citation:
Wang, Deng-Ke,Liu, Ying,Myers, Evan J.,et al. Effects of Nt-truncation and coexpression of isolated Nt domains on the membrane trafficking of electroneutral Na+/HCO3- cotransporters[J]. SCIENTIFIC REPORTS,2015,5.
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