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学科主题口腔医学
The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro
Han, J.; Meng, H. X.; Tang, J. M.; Li, S. L.; Tang, Y.; Chen, Z. B.
刊名CELL PROLIFERATION
2007-04-01
40期:2页:241-252
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]GROWTH-FACTOR-BETA ; OSTEOGENIC DIFFERENTIATION ; BONE ALLOGRAFT ; STEM-CELLS ; REGENERATION ; ENHANCEMENT ; DEFECTS ; COMBINATION ; INVOLVEMENT ; MIGRATION
英文摘要

Objectives: The use of platelets and platelet products has become increasingly popular clinically as a means of accelerating endosseous wound healing. It is likely that growth factors released by activated platelets at the site of injury play a role in periodontal regeneration by regulating cellular activity. The purpose of this study was to evaluate the biological effects of platelet-rich plasma (PRP) on human periodontal ligament cells (hPDLCs) in vitro. Materials and methods: Primary cultures of hPDLCs were obtained from healthy premolars. PRP was isolated by two-step centrifugation. Two main growth factors present in the thrombin-activated PRP (platelet-derived growth factor [PDGF-AB] and transforming growth factor-beta 1 [TGF-beta 1]) were evaluated using ELISA assay. Activated PRP or the combination of recombined human TGF-beta 1 (rhTGF-beta 1) and PDGF-AB (rhPDGF-AB) were added to hPDLCs in different concentrations to assess cell proliferation and osteogenic differentiation. Results: PRP contained high levels of TGF-beta 1 and PDGF-AB. Cell attachment, proliferation and ALP activity were enhanced by addition of PRP or rhTGF-beta 1 and rhPDGF-AB combination to the cell cultures, while the stimulatory potency of PRP was much greater than the latter. These stimulatory effects presented in a dose-dependant manner, it seemed that PRP with 50 similar to 100ng/ml TGF-beta 1 was an ideal concentration. Conclusions: PRP can enhance hPDLC adhesion, proliferation and induce the differentiation of hPDLC into mineralized tissue formation cell; thereby contribute to the main processes of periodontal tissue regeneration. For economical and biological reasons, PRP has more clinical beneficial than analogous growth factors.

语种英语
WOS记录号WOS:000244794400008
引用统计
被引频次:58[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57960
专题北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Dept Histol & Embryol, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Res Lab Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Han, J.,Meng, H. X.,Tang, J. M.,et al. The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro[J]. CELL PROLIFERATION,2007,40(2):241-252.
APA Han, J.,Meng, H. X.,Tang, J. M.,Li, S. L.,Tang, Y.,&Chen, Z. B..(2007).The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro.CELL PROLIFERATION,40(2),241-252.
MLA Han, J.,et al."The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro".CELL PROLIFERATION 40.2(2007):241-252.
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