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学科主题: 基础医学
题名:
Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2 alpha
作者: Zhang, Yan2; Li, Yin2,3; Shibahara, Shigeki2; Takahashi, Kazuhiro1,2
关键词: adrenomedullin ; AP-2 ; transcription ; SP1
刊名: PEPTIDES
发表日期: 2008-03-01
DOI: 10.1016/j.peptides.2007.07.015
卷: 29, 期:3, 页:465-472
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: TRANSCRIPTION FACTOR AP-2 ; MICE LACKING ; ADIPOCYTE DIFFERENTIATION ; ENDOTHELIAL-CELLS ; EPITHELIAL-CELLS ; CANCER CELLS ; EXPRESSION ; INDUCTION ; SECRETION ; PEPTIDE
英文摘要:

Adrenomedullin (AM) is a potent vasodilator peptide, which is ubiquitously expressed and has various biological actions, such as proliferative action and anti-oxidative stress action. AM expression is induced by various stresses, such as hypoxia and inflammatory cytokines, and during cell differentiation. The human AM gene promoter region (-70/-29) contains binding sites for stimulatory protein 1 (Sp1) and activator protein-2 alpha (AP-2 alpha), and has been shown to be important for the AM gene expression during cell differentiation to macrophages or adipocytes. We here show that Sp1 and AP-2 alpha synergistically activate the AM gene promoter. Co-transfection of the reporter plasmid containing the AM promoter region (-103/-29) with Sp1 and AP-2 alpha. expression plasmids showed that Sp1 and AP-2 alpha synergistically increased the promoter activity in HeLa cells. Sp1 or AP-2 alpha alone caused only small increases in the promoter activity. EMSA showed that Sp1 bound to the promoter region (-70/-29), whereas AP-2 alpha bound to a more upstream promoter region (-103/-71). Thus, the synergistic activation of the human AM gene promoter by Sp1 and AP-2 alpha may be mediated by the binding of Sp1 to the promoter region (-70/-29) and the interaction with AP-2 alpha, which binds to the promoter region (-103/-71). (c) 2007 Published by Elsevier Inc.

语种: 英语
WOS记录号: WOS:000254912600019
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57961
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Aoba Ku, Sendai, Miyagi 9808575, Japan
2.Tohoku Univ, Sch Hlth Sci, Dept Mol Biol & Appl Physiol, Sendai, Miyagi 9808575, Japan
3.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Yan,Li, Yin,Shibahara, Shigeki,et al. Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2 alpha[J]. PEPTIDES,2008,29(3):465-472.
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