学科主题基础医学
Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2 alpha
Zhang, Yan2; Li, Yin2,3; Shibahara, Shigeki2; Takahashi, Kazuhiro1,2
关键词adrenomedullin AP-2 transcription SP1
刊名PEPTIDES
2008-03-01
DOI10.1016/j.peptides.2007.07.015
29期:3页:465-472
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]TRANSCRIPTION FACTOR AP-2 ; MICE LACKING ; ADIPOCYTE DIFFERENTIATION ; ENDOTHELIAL-CELLS ; EPITHELIAL-CELLS ; CANCER CELLS ; EXPRESSION ; INDUCTION ; SECRETION ; PEPTIDE
英文摘要

Adrenomedullin (AM) is a potent vasodilator peptide, which is ubiquitously expressed and has various biological actions, such as proliferative action and anti-oxidative stress action. AM expression is induced by various stresses, such as hypoxia and inflammatory cytokines, and during cell differentiation. The human AM gene promoter region (-70/-29) contains binding sites for stimulatory protein 1 (Sp1) and activator protein-2 alpha (AP-2 alpha), and has been shown to be important for the AM gene expression during cell differentiation to macrophages or adipocytes. We here show that Sp1 and AP-2 alpha synergistically activate the AM gene promoter. Co-transfection of the reporter plasmid containing the AM promoter region (-103/-29) with Sp1 and AP-2 alpha. expression plasmids showed that Sp1 and AP-2 alpha synergistically increased the promoter activity in HeLa cells. Sp1 or AP-2 alpha alone caused only small increases in the promoter activity. EMSA showed that Sp1 bound to the promoter region (-70/-29), whereas AP-2 alpha bound to a more upstream promoter region (-103/-71). Thus, the synergistic activation of the human AM gene promoter by Sp1 and AP-2 alpha may be mediated by the binding of Sp1 to the promoter region (-70/-29) and the interaction with AP-2 alpha, which binds to the promoter region (-103/-71). (c) 2007 Published by Elsevier Inc.

语种英语
WOS记录号WOS:000254912600019
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57961
专题北京大学基础医学院_生理学与病理生理学系
北京大学基础医学院
作者单位1.Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Aoba Ku, Sendai, Miyagi 9808575, Japan
2.Tohoku Univ, Sch Hlth Sci, Dept Mol Biol & Appl Physiol, Sendai, Miyagi 9808575, Japan
3.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yan,Li, Yin,Shibahara, Shigeki,et al. Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2 alpha[J]. PEPTIDES,2008,29(3):465-472.
APA Zhang, Yan,Li, Yin,Shibahara, Shigeki,&Takahashi, Kazuhiro.(2008).Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2 alpha.PEPTIDES,29(3),465-472.
MLA Zhang, Yan,et al."Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2 alpha".PEPTIDES 29.3(2008):465-472.
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