IR@PKUHSC  > 基础医学院  > 心血管所
学科主题基础医学
Wnt-promoted Isl1 expression through a novel TCF/LEF1 binding site and H3K9 acetylation in early stages of cardiomyocyte differentiation of P19CL6 cells
Lu, Huafei1; Li, Yanming1; Wang, Yang1; Liu, Yinan1,2,3; Wang, Weiping1; Jia, Zhuqing1; Chen, Ping1; Ma, Kangtao1; Zhou, Chunyan1
关键词Wnt/beta-catenin Islet 1 Cardiomyocyte Differentiation P19cl6 Cells H3k9 Acetylation
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2014-06-01
DOI10.1007/s11010-014-2001-y
391期:1-2页:183-192
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者National Natural Sciences Foundation of China ; Leading Academic Discipline Project of Beijing Education Bureau ; 111 Project of Ministry of Education of China ; National Natural Sciences Foundation of China ; Leading Academic Discipline Project of Beijing Education Bureau ; 111 Project of Ministry of Education of China
研究领域[WOS]Cell Biology
关键词[WOS]2ND HEART FIELD ; BETA-CATENIN ; CARDIOVASCULAR PROGENITORS ; CARDIAC PROGENITORS ; WNT/BETA-CATENIN ; DOWN-REGULATION ; MOUSE HEART ; STEM-CELLS ; ISLET-1 ; LINEAGES
英文摘要

Islet 1 (ISL1), a marker of second heart field progenitors, plays a crucial role in cardiomyocyte differentiation and proliferation. However, little is known about transcriptional regulating mechanisms on Isl1 gene expression. Recent studies have demonstrated that Wnt/beta-catenin signaling regulates Isl1 expression during heart development. However, the detailed mechanisms still remain unclear. In the present study performed during differentiation of P19CL6 into cardiomyocytes, we explored the underlying regulating mechanisms on Wnt/beta-catenin-mediated Isl1 expression after we first confirmed that Wnt/beta-catenin signaling promoted cardiomyocyte differentiation partly through Isl1 activation. We found a novel TCF/LEF1 binding site that was located 2300 bp upstream of the Isl1 ATG. Furthermore, Wnt/beta-catenin signaling upregulated histone H3K9 acetylation on TCF/LEF1 binding sites on the Isl1 promoter, resulting in upregulation of Isl1 expression. This Wnt-mediated H3K9 acetylation on the Isl1 promoter was modulated by the acetyltransferase CREB-binding protein (CBP), instead of p300, through interaction with beta-catenin. Collectively, these results suggest that in early stages of cardiomyocyte differentiation Wnt/beta-catenin signaling promotes Isl1 expression via two ways: a novel TCF/LEF1 binding site and H3K9 acetylation conducted by CBP on the Isl1 promoter. To our knowledge, this is the first study reporting Wnt/beta-catenin-regulated H3K9 acetylation on promoters of its target genes. And this study gives new insights into transcriptional regulating mechanisms of Wnt-mediated Isl1 expression during cardiomyocyte differentiation.

语种英语
所属项目编号81370236 ; 30871253 ; 90919022 ; 81170713 ; 30400242 ; B07001
资助者National Natural Sciences Foundation of China ; Leading Academic Discipline Project of Beijing Education Bureau ; 111 Project of Ministry of Education of China ; National Natural Sciences Foundation of China ; Leading Academic Discipline Project of Beijing Education Bureau ; 111 Project of Ministry of Education of China
WOS记录号WOS:000335570400020
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57977
专题基础医学院_心血管所
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Minist Educ China, Key Lab Mol Cardiovasc Sci, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Lu, Huafei,Li, Yanming,Wang, Yang,et al. Wnt-promoted Isl1 expression through a novel TCF/LEF1 binding site and H3K9 acetylation in early stages of cardiomyocyte differentiation of P19CL6 cells[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2014,391(1-2):183-192.
APA Lu, Huafei.,Li, Yanming.,Wang, Yang.,Liu, Yinan.,Wang, Weiping.,...&Zhou, Chunyan.(2014).Wnt-promoted Isl1 expression through a novel TCF/LEF1 binding site and H3K9 acetylation in early stages of cardiomyocyte differentiation of P19CL6 cells.MOLECULAR AND CELLULAR BIOCHEMISTRY,391(1-2),183-192.
MLA Lu, Huafei,et al."Wnt-promoted Isl1 expression through a novel TCF/LEF1 binding site and H3K9 acetylation in early stages of cardiomyocyte differentiation of P19CL6 cells".MOLECULAR AND CELLULAR BIOCHEMISTRY 391.1-2(2014):183-192.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
art%3A10.1007%2Fs110(800KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lu, Huafei]的文章
[Li, Yanming]的文章
[Wang, Yang]的文章
百度学术
百度学术中相似的文章
[Lu, Huafei]的文章
[Li, Yanming]的文章
[Wang, Yang]的文章
必应学术
必应学术中相似的文章
[Lu, Huafei]的文章
[Li, Yanming]的文章
[Wang, Yang]的文章
相关权益政策
暂无数据
收藏/分享
文件名: art%3A10.1007%2Fs11010-014-2001-y.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。