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学科主题: 药学
题名:
Chemical analysis of the Tibetan herbal medicine Carduus acanthoides by UPLC/DAD/qTOF-MS and simultaneous determination of nine major compounds
作者: Li, Ru; Liu, Sui-ku; Song, Wei; Wang, Yuan; Li, Yan-jiao; Qiao, Xue; Liang, Hong; Ye, Min
刊名: ANALYTICAL METHODS
发表日期: 2014
DOI: 10.1039/c4ay01138d
卷: 6, 期:18, 页:7181-7189
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]: Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]: TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; LC-MSN ; FLAVONOIDS ; IDENTIFICATION ; PLANTS ; GLYCOSIDES ; AGLYCONES ; LEAVES ; ACIDS
英文摘要:

Carduus acanthoides is a traditional Tibetan herbal medicine for the treatment of hematemesis, hematuria, and menorrhagia. Despite its multiple biological activities, comprehensive chemical analysis of this herb has not been reported, so far. In this study, chemical constituents of C. acanthoides were analyzed by ultra-performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry (UPLC/DAD/qTOF-MS). The methanol extract of C. acanthoides was separated on an ACQUITY HSS T3 column (1.8 mu m, 150 mm x 2.1 mm) and eluted with acetonitrile-water (containing 0.1% formic acid). Constituents were identified by UV and HRMS in the negative ion mode. A total of 69 compounds were identified from C. acanthoides, including 35 flavonoids, 17 quinic acids, 7 phenolic acid glycosides, 5 phenylethanoid glycosides, 3 coumarins, and 2 phenolic acids. Among them, 31 compounds were unambiguously characterized by comparing with reference standards. Furthermore, a UPLC/UV (340 nm) method was established to simultaneously determine 3 quinic acids and 6 flavonoids, including neochlorogenic acid (9), chlorogenic acid (16), cryptochlorogenic acid (18), kaempferol 3-O-rhamnoside-7-O-glucoside (32), luteolin 7-O-glucosyl-(1 -> 2)-glucoside (34), luteolin 7-O-glucoside (38), luteolin 7-O-6 ′′′-O-acetyl-glucosyl-(1 -> 2)-glucoside (44), kaempferol 3-O-rhamnoside (55), and chrysoeriol 7-O-6 ′′′-O-acetyl-glucosyl-(1 -> 2)-glucoside (56). All analytes showed good linearity (r(2) >= 0.998) with an LOD of 0.077-0.120 mu g mL(-1). These nine compounds accounted for 2.1-3.5 parts per thousand of the herb.

语种: 英语
所属项目编号: 81222054 ; NCET-11-0019
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education
WOS记录号: WOS:000341080900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57978
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Li, Ru,Liu, Sui-ku,Song, Wei,et al. Chemical analysis of the Tibetan herbal medicine Carduus acanthoides by UPLC/DAD/qTOF-MS and simultaneous determination of nine major compounds[J]. ANALYTICAL METHODS,2014,6(18):7181-7189.
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