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学科主题基础医学
Bicistronic adenovirus-mediated gene transfer of CTLA4Ig gene and CD40Ig gene result in indefinite survival of islet xenograft
Jin, YZ; Xie, SS
刊名TRANSPLANTATION PROCEEDINGS
2003-12-01
DOI10.1016/j.tranproceed.2003.10.064
35期:8页:3165-3166
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Surgery ; Transplantation
研究领域[WOS]Immunology ; Surgery ; Transplantation
关键词[WOS]LIGAND
英文摘要

Blockade of CD40-CD154 costimulatory pathway in mice and primates with anti-CD154 monoclonal antibodies results in prolonged survival of vascularized organs and islet grafts. CD40Ig, a recombinant fusion protein comprised of the extracellular domain of human CD40 molecule in frame fused with the site-mutated human IgG1 Fc region, abrogated the cognate interaction of CD40-CD154 pathway by binding the CD154 molecule. In this study, replication-defective adenovirus containing the CD40Ig gene was prepared by homologous recombination and used to infect freshly isolated islets from LEW rats (RT-1(1)) in vitro using a titered dose. The islet transfectants (500 per recipient) were transplanted under the left kidney capsule of streptozocin-rendered diabetic C57BL/6 mouse recipient (H-2(b)). The mean survival time of AdCD40Ig-transfected islet grafts was significantly prolonged, while mock-infected grafts and AdEGFP-transfected grafts were rejected in normal fashion. Additionally, dose-dependent prolongation of islet graft survival was observed in mice receiving AdCD40Ig-transfected grafts. In conclusion, local production of Cd40Ig via adenoviral-mediated gene transfer induced dose-dependent prolongation of LEW --> Balb-c islet xenografts.

语种英语
WOS记录号WOS:000187576900087
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57988
Collection北京大学基础医学院_免疫学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Jin, YZ,Xie, SS. Bicistronic adenovirus-mediated gene transfer of CTLA4Ig gene and CD40Ig gene result in indefinite survival of islet xenograft[J]. TRANSPLANTATION PROCEEDINGS,2003,35(8):3165-3166.
APA Jin, YZ,&Xie, SS.(2003).Bicistronic adenovirus-mediated gene transfer of CTLA4Ig gene and CD40Ig gene result in indefinite survival of islet xenograft.TRANSPLANTATION PROCEEDINGS,35(8),3165-3166.
MLA Jin, YZ,et al."Bicistronic adenovirus-mediated gene transfer of CTLA4Ig gene and CD40Ig gene result in indefinite survival of islet xenograft".TRANSPLANTATION PROCEEDINGS 35.8(2003):3165-3166.
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