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学科主题: 临床医学
题名:
Hematopoietic stem cells mobilized by granulocyte colony-stimulating factor partly contribute to liver graft regeneration after partial orthotopic liver transplantation
作者: Liu, F; Pan, XB; Chen, GD; Jiang, D; Cong, X; Fei, R; Wei, L
刊名: LIVER TRANSPLANTATION
发表日期: 2006-07-01
DOI: 10.1002/lt.20822
卷: 12, 期:7, 页:1129-1137
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Surgery ; Transplantation
研究领域[WOS]: Gastroenterology & Hepatology ; Surgery ; Transplantation
关键词[WOS]: BONE-MARROW-CELLS ; PERIPHERAL-BLOOD ; IN-VIVO ; ENDOTHELIAL-CELLS ; HEPATOCYTES ; FUSION ; DONOR ; MICE ; SIZE ; RAT
英文摘要:

On the basis of the recently recognized potential of hematopoietic stem cells (HSCs) to give rise to hepatocytes, we investigated whether HSCs mobilized by granulocyte colony-stimulating factor (G-CSF) or G-CSF per se could contribute to faster recovery and promote tissue reparation after rats′ (cross-sex) partial orthotopic liver transplantation (PLTx). Sex-mismatched (female to male) syngeneic rat PLTx was established. The recipients were repeatedly administrated recombinant G-CSF for 5 consecutive days before (G-CSF + PLTx group) and after PLTx (PLTx + G-CSF group). Compared with those in PLTx group, CD34(+) cells in peripheral blood and portal tract region increased from day 1 to 7 after transplantation in G-CSF + PLTx group and from day 3 to 14 after transplantation in PLTx + G-CSF group, respectively, which suggested that CD34(+) HSCs were mobilized and migrated into liver graft. Compared with that in G-CSF + PLTx and PLTx groups, there was a higher survival rate in the PLTx + G-CSF group. On day 3 after surgery, the level of aspartate aminotransferase and alanine aminotransferase were lower, whereas the mitosis index, proliferating cell nuclear antigen-positive nuclei, bromodeoxyuridine (BrdU) incorporation, and graft-to-recipient weight ratio were higher in the PLTx + G-CSF group. In contrast, these parameters had no significant difference between G-CSF + PLTx and PLTx groups. To define the origin of proliferating cells reconstituting liver after injury, sry(+) (sex-determining region for Y chromosome) and sry(+)/cytokeratin 19(+) (CK19) cells were quantitated. Higher percentage of sry+ and sry(+)/CK19(+) cells in PLTx + G-CSF was detected than in G-CSF + PLTx groups on day 14 after surgery, although the liver engraftment rate still remained rather low. Some of the sry+/CK19(+) cells in the portal tract areas were similar to hepatic oval cells/cholangiocytes. In conclusion, G-CSF administration after PLTx greatly improved survival rate and liver regeneration of partial graft, partly by its mobilizing HSCs into the injured liver to differentiate into hepatocytes through hepatic oval cells′/cholangiocytes′ engraftment.

语种: 英语
WOS记录号: WOS:000238711700017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57992
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing, Peoples R China

Recommended Citation:
Liu, F,Pan, XB,Chen, GD,et al. Hematopoietic stem cells mobilized by granulocyte colony-stimulating factor partly contribute to liver graft regeneration after partial orthotopic liver transplantation[J]. LIVER TRANSPLANTATION,2006,12(7):1129-1137.
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