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学科主题基础医学
Defective Differentiation of Adipose Precursor Cells from Lipodystrophic Mice Lacking Perilipin 1
Lyu, Ying; Su, Xueying; Deng, Jingna; Liu, Shangxin; Zou, Liangqiang; Zhao, Xiaojing; Wei, Suning; Geng, Bin; Xu, Guoheng
刊名PLOS ONE
2015-02-19
DOI10.1371/journal.pone.0117536
10期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]FAMILIAL PARTIAL LIPODYSTROPHY ; PRIMARY RAT ADIPOCYTES ; LIPID DROPLETS ; IN-VIVO ; GENERALIZED LIPODYSTROPHY ; PROGENITOR CELLS ; GENE-EXPRESSION ; UP-REGULATION ; TISSUE ; LIPOLYSIS
英文摘要

Perilipin 1 (Plin1) localizes at the surface of lipid droplets to regulate triglyceride storage and hydrolysis in adipocytes. Plin1 defect leads to low adiposity in mice and partial lipodystrophy in human. This study investigated the roles of Plin1 in adipocyte differentiation. Plin1 null (-/-) mice showed plenty of multilocular adipocytes and small unilocular adipocytes in adipose tissue, along with lack of a subpopulation of adipose progenitor cells capable of in vivo adipogenesis and along with downregulation of adipogenic pathway. Before initiation of differentiation, adipose stromal-vascular cells (SVCs) from Plin1-/-mice already accumulated numerous tiny lipid droplets, which increased in number and size during the first 12-h induction but thereafter became disappeared at day 1 of differentiation. The adipogenic signaling was dysregulated despite protein level of PPAR. was near normal in Plin1-/-SVCs like in Plin1-/-adipose tissue. Heterozygous Plin1+/-SVCs were able to develop lipid droplets, with both the number and size more than in Plin1-/-SVCs but less than in Plin1+/+ SVCs, indicating that Plin1 haploinsufficiency accounts for attenuated adipogenesis. Aberrant lipid droplet growth and differentiation of Plin1-/-SVCs were rescued by adenoviral Plin1 expression and were ameliorated by enhanced or prolonged adipogenic stimulation. Our finding suggests that Plin1 plays an important role in adipocyte differentiation and provides an insight into the pathology of partial lipodystrophy in patients with Plin1 mutation.

语种英语
所属项目编号91439119 ; 7152080 ; 2012CB517505 ; 2012CB517806
资助者National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program of China
WOS记录号WOS:000350062300023
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57999
专题基础医学院
作者单位Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Lyu, Ying,Su, Xueying,Deng, Jingna,et al. Defective Differentiation of Adipose Precursor Cells from Lipodystrophic Mice Lacking Perilipin 1[J]. PLOS ONE,2015,10(2).
APA Lyu, Ying.,Su, Xueying.,Deng, Jingna.,Liu, Shangxin.,Zou, Liangqiang.,...&Xu, Guoheng.(2015).Defective Differentiation of Adipose Precursor Cells from Lipodystrophic Mice Lacking Perilipin 1.PLOS ONE,10(2).
MLA Lyu, Ying,et al."Defective Differentiation of Adipose Precursor Cells from Lipodystrophic Mice Lacking Perilipin 1".PLOS ONE 10.2(2015).
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