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学科主题: 临床医学
题名:
Role of major histocompatibility complex class I-related molecules A*A5 center dot 1 allele in ulcerative colitis in Chinese patients
作者: Lue, Min3; Xia, Bing1,2,4; Ge, Liuqing2; Li, Yi2; Zhao, Jie2; Chen, Fan4; Zhou, Feng2; Zhang, Xiaolian4; Tan, Jinquan4
关键词: major histocompatibility complex class I chain-related gene A ; microsatellite polymorphism ; mutation ; natural killer cell ; ulcerative colitis
刊名: IMMUNOLOGY
发表日期: 2009-09-01
DOI: 10.1111/j.1365-2567.2008.02953.x
卷: 128, 期:1, 页:e230-e236
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: INFLAMMATORY-BOWEL-DISEASE ; CHAIN-RELATED GENE ; TRIPLET REPEAT POLYMORPHISM ; MIC-A GENE ; MICROSATELLITE POLYMORPHISM ; TRANSMEMBRANE REGION ; CYTO-TOXICITY ; T-CELLS ; POPULATION ; EPIDEMIOLOGY
英文摘要:

P>The major histocompatibility complex (MHC) class I-related molecules A (MICA) is a stress-inducible cell surface antigen that is recognized by intestinal epithelial V delta 1 gamma delta T cells, natural killer (NK) cells and CD8(+) T cells with NKG2D receptor participating in the immunological reaction in the intestinal mucosa. The present study aimed to investigate the functions of the MICA*A5.1 allele in the development of ulcerative colitis (UC) in the Chinese population. The microsatellite polymorphisms of MICA were genotyped in 124 unrelated Chinese patients with UC and 172 ethnically matched healthy controls using a semiautomatic fluorescently labelled polymerase chain reaction. MICA*A5.1-expressing Raji cells were generated by gene transfection. Cytotoxicity of NK cells to Raji cells expressing different MICA molecules was detected using the lactate dehydrogenase method. Soluble MICA in the culture supernatant was detected by enzyme-linked immunosorbent assay. The frequency of MICA*A5.1 was significantly higher in UC patients compared with the healthy controls (29 center dot 0% versus 17 center dot 4%, P = 0 center dot 001, corrected P = 0 center dot 005, OR = 1 center dot 936, 95% CI 1 center dot 310-2 center dot 863) and the frequency of a MICA*A5.1/A5.1 homozygous genotype was increased in UC patients (18 center dot 5% versus 7% in healthy controls, P = 0 center dot 0032, corrected P = 0 center dot 048, OR = 3 center dot 036, 95% CI 1 center dot 447-6 center dot 372). Raji cells with MICA*A5.1 expression produced more soluble MICA (t = 5 center dot 75, P < 0 center dot 01) than Raji cells with full-length MICA expression in culture supernatant. Raji cells with MICA*A5.1 expression were more resistant to killing by NK cells than Raji cells with full-length MICA expression. The MICA*A5.1 allele and MICA*A5.1/A5.1 genotype are significantly associated with Chinese UC patients in central China. MICA*A5.1 may play a role in the development of UC by producing more soluble MICA and resistance to NK cells.

语种: 英语
所属项目编号: 30570834
项目资助者: Natural Science Foundation of China
WOS记录号: WOS:000268703800008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58006
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Zhongnan Hosp, Digest Dis Res Ctr, Wuhan, Peoples R China
2.Wuhan Univ, Zhongnan Hosp, Dept Gastroenterol, Wuhan 430071, Hubei Province, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Internal Med, Beijing 100871, Peoples R China
4.Wuhan Univ, Sch Med, Key Lab Allergy & Immune Related Dis, Wuhan 430071, Hubei Province, Peoples R China

Recommended Citation:
Lue, Min,Xia, Bing,Ge, Liuqing,et al. Role of major histocompatibility complex class I-related molecules A*A5 center dot 1 allele in ulcerative colitis in Chinese patients[J]. IMMUNOLOGY,2009,128(1):e230-e236.
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