IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Cytotoxic Activity of Anticancer Drugs on Hepatocellular Carcinoma Cells in Hypoxic-Hyponutritional Culture
Li, Qiang; Zhu, Lin-Zhong; Yang, Ren-Jie; Zhu, Xu
关键词Hepatocellular Carcinoma Transcatheter Arterial Chemoembolization Chemosensitivity Test Mtt Assay Hypoxia
刊名INTERNATIONAL SURGERY
2014-11-01
DOI10.9738/INTSURG-D-14-00073.1
99期:6页:745-752
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Surgery
研究领域[WOS]Surgery
关键词[WOS]TRANSCATHETER ARTERIAL CHEMOEMBOLIZATION ; IN-VITRO ; INFUSION CHEMOTHERAPY ; TUMOR-GROWTH ; MTT ASSAY ; CANCER ; EMBOLIZATION ; CHOLANGIOCARCINOMA ; EXPERIENCE ; THERAPIES
英文摘要

To investigate which anticancer drugs and combination of dual drugs could further promote the inhibition of cell growth in vitro against HCC cell line (HepG2) in the hypoxic and hyponutritional culture medium(HHCM) mimicked the different scenarios of transcatheter arterial chemoembolization (TACE). The cells of hepatocellular carcinoma (HCC) treated by TACE suffered various hypoxia and hyponutrition. The cells were treated for 2 hours, 4 hours, 6 hours, and 24 hours, respectively, using 10 drugs including epirubicin (EPI), cisplatin (DDP), mitomycin-C (MMC), oxaliplatin (OXA), hydroxycamptothecin (HCPT), 5-fluorouracil (5-FU), gemcitabine (GEM), docetaxel (DTX), thiotepa (TSPA), and pemetrexed disodium (PEM) in 4 concentrations of HHCM (5%, 10%, 25%, and 50%, respectively) mimicking the scenario of TACE and were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells treated with combinations of dual drugs for 24 hours were also tested. The sensitive drugs with inhibition rates more than 30% were EPI, MMC, HCPT, OXA, and PEM in 4 types of HHCMs. The sensitivity of the cells to treatment with drugs for 24 hours was significantly higher than the sensitivity of the cells to treatment with drugs for 2 hours in 5%, 10%, and 25% HHCM. The sensitivity of the combination of dual drugs was no more than the sensitivity of the single drug with higher sensitivity in 4 concentrations of HHCM. EPI, MMC, HCPT, OXA, and PEM exhibited cytotoxic activity against HepG2 cells in various hypoxia and hyponutrition states. Prolonging the time of exposure could increase the sensitivity of drug, and the combination of dual drugs cannot enhance the cytotoxic effect.

语种英语
WOS记录号WOS:000345906800013
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58009
专题北京大学临床肿瘤学院
北京大学第一临床医学院_整形烧伤外科
北京大学临床肿瘤学院_介入治疗科
作者单位Peking Univ, Canc Hosp, Dept Intervent Radiol, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Li, Qiang,Zhu, Lin-Zhong,Yang, Ren-Jie,et al. Cytotoxic Activity of Anticancer Drugs on Hepatocellular Carcinoma Cells in Hypoxic-Hyponutritional Culture[J]. INTERNATIONAL SURGERY,2014,99(6):745-752.
APA Li, Qiang,Zhu, Lin-Zhong,Yang, Ren-Jie,&Zhu, Xu.(2014).Cytotoxic Activity of Anticancer Drugs on Hepatocellular Carcinoma Cells in Hypoxic-Hyponutritional Culture.INTERNATIONAL SURGERY,99(6),745-752.
MLA Li, Qiang,et al."Cytotoxic Activity of Anticancer Drugs on Hepatocellular Carcinoma Cells in Hypoxic-Hyponutritional Culture".INTERNATIONAL SURGERY 99.6(2014):745-752.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Qiang]的文章
[Zhu, Lin-Zhong]的文章
[Yang, Ren-Jie]的文章
百度学术
百度学术中相似的文章
[Li, Qiang]的文章
[Zhu, Lin-Zhong]的文章
[Yang, Ren-Jie]的文章
必应学术
必应学术中相似的文章
[Li, Qiang]的文章
[Zhu, Lin-Zhong]的文章
[Yang, Ren-Jie]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。