IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科
学科主题临床医学
Adrenomedullin impairs the profibrotic effects of transforming growth factor-beta 1 through recruiting Smad6 protein in human renal tubular cells
Huang, HC; Ma, CY; Yang, M; Tang, CS; Wang, HY
关键词Adrenomedullin Transforming Growth Factor-beta Fibrosis Kidney Smad
刊名CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
2005
DOI10.1159/000083644
15期:1-4页:117-124
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Physiology
研究领域[WOS]Cell Biology ; Physiology
关键词[WOS]TGF-BETA ; GROWTH-FACTOR ; SIGNALING PATHWAY ; PLASMA ; RECEPTOR ; KIDNEY ; PROLIFERATION ; ACTIVATION ; EXPRESSION ; FAILURE
英文摘要

Adrenomedullin ( AM) was originally identified as a vasodilator peptide, and has recently been shown to be an antiproliferative factor in renal mesangial cells, suggesting that adrenomedullin may impair the progression of glomerulosclerosis. This study was to investigate the effect of adrenomedullin on transforming growth factor-beta(1) ( TGF-beta(1))- stimulated cell growth, synthesis of extracellular matrix ( ECM) components and the related molecular mechanism in a human tubular epithelial cell line HK- 2. TGF-beta(1) inhibited cell proliferation induced by fetal bovine serum, but neither AM itself affectted cell proliferation, nor did AM influence TGF-beta(1)- caused cell growth arrest. However, AM beginning at 10(-8) M alleviated the action of TGF-beta(1)- stimulated cellular collagen synthesis and secretion of fibronectin into cell culture supernatant. Activation of Smad proteins is known to be the key signaling pathway of the profibrotic effect of TGF-beta(1), AM at 10(-8) M exerted no effect on TGF-beta(1)- induced Smad2 phosphorylation, but prevented the suppression of the inhibitory Smad6 protein by TGF-beta(1) and restored Smad2- Samd6 complex formation. Our results suggest that AM can attenuate TGF- beta(1)-mediated renal tubulointerstitial ECM turnover via an antagonistic mechanism of inhibitory Smad in TGF-beta(1)-elicited signaling. Copyright (C) 2005 S. Karger AG, Basel.

语种英语
WOS记录号WOS:000226588000012
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58011
Collection北京大学第一临床医学院_肾脏内科
作者单位1.Peking Univ, Inst Cardiovasc Res, Beijing, Peoples R China
2.Peking Univ, Inst Nephrol, Beijing, Peoples R China
3.Peking Univ, Hosp 1, Div Nephrol, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Huang, HC,Ma, CY,Yang, M,et al. Adrenomedullin impairs the profibrotic effects of transforming growth factor-beta 1 through recruiting Smad6 protein in human renal tubular cells[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2005,15(1-4):117-124.
APA Huang, HC,Ma, CY,Yang, M,Tang, CS,&Wang, HY.(2005).Adrenomedullin impairs the profibrotic effects of transforming growth factor-beta 1 through recruiting Smad6 protein in human renal tubular cells.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,15(1-4),117-124.
MLA Huang, HC,et al."Adrenomedullin impairs the profibrotic effects of transforming growth factor-beta 1 through recruiting Smad6 protein in human renal tubular cells".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 15.1-4(2005):117-124.
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