IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Human breast carcinoma xenografts in nude mice
Li, ZH; Huang, XF; Li, JY; Ke, Y; Yang, LG; Wang, YX; Yao, LH; Lu, YW
关键词Breast Neoplasm Xenograft Micrometastasis Microsatellite Dna
刊名CHINESE MEDICAL JOURNAL
2002-02-01
115期:2页:222-226
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]ATHYMIC MICE ; HUMAN-TUMORS ; CANCER ; METASTASIS ; GROWTH ; MOUSE ; MODEL
英文摘要

Objective To investigate spontaneous metastasis, micrometastasis and genetic stability in human breast carcinoma xenografts in nude mice.

Methods Intact tissue from surgical specimens from breast carcinoma patients was xenografted into nude mice and transplanted from generation to generation. Cells from the xenografts were cultured in vitro and retransplanted into nude mice. Microsatellite DNA in the genome of human breast carcinomas, xenotransplanted tumors and metastases in nude mice were analyzed at three microsatellite loci.

Results The tumorigenicity of orthotopic xenotransplantation was 88.6% (31/35), with a metastatic rate of 41.9% (13/31). Cells from xenotransplants were successfully cultured in vitro. The taking rate of retransplantation into nude mice and the spontaneous lung metastasis rate were both 100% (10/10). Microsatellite DNA sequences in the genome of xenotransplanted tumors and metastases in nude mice were identical with that of the original human breast carcinoma at three microsatellite loci.

Conclusions Tumorigenicity and metastatic potential can be improved in human breast carcinoma xenografts using intact fresh tumor tissue and orthotopic grafts. Xenotransplanted tumors and tumors after serial passage maintained the genetic stability. The detection of microsatellite DNA may identify micrometastases in a nude mouse model.

语种英语
WOS记录号WOS:000174163000015
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58020
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Clin Sch Oncol, Beijing Inst Canc Res, Dept Surg, Beijing 100036, Peoples R China
2.Peking Univ, Clin Sch Oncol, Beijing Inst Canc Res, Dept Pathol, Beijing 100036, Peoples R China
3.Peking Univ, Clin Sch Oncol, Beijing Inst Canc Res, Lab Cytogenet, Beijing 100036, Peoples R China
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GB/T 7714
Li, ZH,Huang, XF,Li, JY,et al. Human breast carcinoma xenografts in nude mice[J]. CHINESE MEDICAL JOURNAL,2002,115(2):222-226.
APA Li, ZH.,Huang, XF.,Li, JY.,Ke, Y.,Yang, LG.,...&Lu, YW.(2002).Human breast carcinoma xenografts in nude mice.CHINESE MEDICAL JOURNAL,115(2),222-226.
MLA Li, ZH,et al."Human breast carcinoma xenografts in nude mice".CHINESE MEDICAL JOURNAL 115.2(2002):222-226.
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