北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Human breast carcinoma xenografts in nude mice
作者: Li, ZH; Huang, XF; Li, JY; Ke, Y; Yang, LG; Wang, YX; Yao, LH; Lu, YW
关键词: breast neoplasm ; xenograft ; micrometastasis ; microsatellite DNA
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2002-02-01
卷: 115, 期:2, 页:222-226
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: ATHYMIC MICE ; HUMAN-TUMORS ; CANCER ; METASTASIS ; GROWTH ; MOUSE ; MODEL
英文摘要:

Objective To investigate spontaneous metastasis, micrometastasis and genetic stability in human breast carcinoma xenografts in nude mice.

Methods Intact tissue from surgical specimens from breast carcinoma patients was xenografted into nude mice and transplanted from generation to generation. Cells from the xenografts were cultured in vitro and retransplanted into nude mice. Microsatellite DNA in the genome of human breast carcinomas, xenotransplanted tumors and metastases in nude mice were analyzed at three microsatellite loci.

Results The tumorigenicity of orthotopic xenotransplantation was 88.6% (31/35), with a metastatic rate of 41.9% (13/31). Cells from xenotransplants were successfully cultured in vitro. The taking rate of retransplantation into nude mice and the spontaneous lung metastasis rate were both 100% (10/10). Microsatellite DNA sequences in the genome of xenotransplanted tumors and metastases in nude mice were identical with that of the original human breast carcinoma at three microsatellite loci.

Conclusions Tumorigenicity and metastatic potential can be improved in human breast carcinoma xenografts using intact fresh tumor tissue and orthotopic grafts. Xenotransplanted tumors and tumors after serial passage maintained the genetic stability. The detection of microsatellite DNA may identify micrometastases in a nude mouse model.

语种: 英语
WOS记录号: WOS:000174163000015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58020
Appears in Collections:北京大学临床肿瘤学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Clin Sch Oncol, Beijing Inst Canc Res, Dept Surg, Beijing 100036, Peoples R China
2.Peking Univ, Clin Sch Oncol, Beijing Inst Canc Res, Dept Pathol, Beijing 100036, Peoples R China
3.Peking Univ, Clin Sch Oncol, Beijing Inst Canc Res, Lab Cytogenet, Beijing 100036, Peoples R China

Recommended Citation:
Li, ZH,Huang, XF,Li, JY,et al. Human breast carcinoma xenografts in nude mice[J]. CHINESE MEDICAL JOURNAL,2002,115(2):222-226.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, ZH]'s Articles
[Huang, XF]'s Articles
[Li, JY]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, ZH]‘s Articles
[Huang, XF]‘s Articles
[Li, JY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace