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学科主题: 基础医学
题名:
Evaluation of estrogenic potential of Shu-Gan-Liang-Xue Decoction by dual-luciferase reporter based bioluminescent measurements in vitro
作者: Zhang, Yi2,3; Li, Ping-Ping1
关键词: Estrogenic activity ; Breast cancer ; Hot flushes ; Dual-luciferase reporter ; Bioluminescent measurements ; Shu-Gan-Liang-Xue Decoction
刊名: JOURNAL OF ETHNOPHARMACOLOGY
发表日期: 2009-11-12
DOI: 10.1016/j.jep.2009.08.016
卷: 126, 期:2, 页:345-349
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]: BREAST-CANCER ; CELLS ; SAIKOSAPONIN ; TAMOXIFEN ; SHIKONIN ; GROWTH ; WOMEN ; TRIAL ; PROLIFERATION ; EXPRESSION
英文摘要:

Ethnopharmacological relevance: Shu-Gan-Liang-Xue Decoction (SGLXD), a cipher prescription of traditional Chinese medicine, has been used to ameliorate hot flushes symptom in breast cancer patients for several decades. However, estrogenic activity of SGLXD is remaining unclear.

Aim of the study: To evaluate the estrogenic potential of SGLXD and each of the component herbs; to investigate the effect of SGLXD on cell viability of MCF-7 cells.

Materials and methods: We evaluated the estrogenic potential of SGLXD and each of the component herbs with dual-luciferase reporter assay and bioluminescent measurements, by using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells. We investigated the effect of SGLXD on cell viability of MCF-7 by MTT assay.

Results: SGLXD and each of the component herbs did not demonstrate estrogenic activity compared to negative control, even at high concentration (p > 0.05). By WIT assay, different concentrations of SGLXD significantly inhibited the growth of MCF-7 cells dose-dependently (p < 0.05).

Conclusions: SGLXD and single herbs do not manifest estrogenic activity, while SGLXD has weak inhibitory effects on MCF-7 cell viability. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 2004-IV15
项目资助者: Key Program Foundation of Beijing TCMs Administration, PR China
WOS记录号: WOS:000272368000023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58025
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Key Lab Carcinogenesis & Translat Res, Dept Integrated Tradit Chinese & Western Med, Minist Educ,Sch Oncol,Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
2.Beijing Univ Chinese Med, Sch Preclin Med, Beijing 100029, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Integrated Tradit Chinese & Western Med, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Yi,Li, Ping-Ping. Evaluation of estrogenic potential of Shu-Gan-Liang-Xue Decoction by dual-luciferase reporter based bioluminescent measurements in vitro[J]. JOURNAL OF ETHNOPHARMACOLOGY,2009,126(2):345-349.
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