IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Synergistic Inhibition of Endochondral Bone Formation by Silencing Hif1 alpha and Runx2 in Trauma-induced Heterotopic Ossification
Lin, Lin1; Shen, Qi2; Leng, Huijie3; Duan, Xiaoning1; Fu, Xin1; Yu, Changlong1
刊名MOLECULAR THERAPY
2011-08-01
DOI10.1038/mt.2011.101
19期:8页:1426-1432
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]HYPOXIA-INDUCIBLE FACTOR ; MESENCHYMAL STEM-CELLS ; OSTEOBLAST DIFFERENTIATION ; SKELETAL DEVELOPMENT ; MEDIATED TRANSFER ; IN-VITRO ; EXPRESSION ; CARTILAGE ; CBFA1 ; SKELETOGENESIS
英文摘要

Angiogenesis and osteogenesis are tightly coupled during bone development. We studied the effect of inhibition of Hif1 alpha and Runt-related protein 2 (Runx2) on the formation of heterotopic ossification (HO). We constructed lentivirus vectors expressing Hif1 alpha small interfering RNA (siRNA) and Runx2 siRNA. The inhibition of Hif1 alpha function impaired osteoblast proliferation while osteoblasts differentiated normally. Osteoblasts lacking Runx2 proliferated normally while the differentiation was impaired. The osteoblast differentiation was significantly inhibited by co-Runx2 and Hif1 alpha siRNA treatment. The formation of HO by inhibiting Runx2 and Hif1 alpha in an animal model induced by Achilles tenotomy was investigated. The results showed that lacking of Runx2 and Hif1 alpha could inhibit HO formation. Inhibition of Hif1 alpha prevented HO formation only at the initial step and inhibition of Runx2 worked both at the initial step and after chondrogenesis. Angiogenesis and the expressions of osteogenic genes were downregulated in the Hif1 alpha siRNA group. We found synergistic inhibition of endochondral bone formation by silencing Hif1 alpha and Runx2. Our study provided new insight into the roles of Hif1 alpha and Runx2 during the processes of endochondral bone formation, and had important implications for the new therapeutic methods to inhibit HO or to enhance bone formation.

语种英语
WOS记录号WOS:000293378500006
项目编号2008B04
资助机构Beijing NOVA ; Beijing Municipal Science and Technology Commission ; Doctoral Program Foundation of Institutions of Higher Education of China
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58028
专题北京大学第三临床医学院_运动医学研究所
北京大学精神卫生研究所_护理部
作者单位1.Peking Univ, Hosp 1, Inst Urol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Lin, Lin,Shen, Qi,Leng, Huijie,et al. Synergistic Inhibition of Endochondral Bone Formation by Silencing Hif1 alpha and Runx2 in Trauma-induced Heterotopic Ossification[J]. MOLECULAR THERAPY,2011,19(8):1426-1432.
APA Lin, Lin,Shen, Qi,Leng, Huijie,Duan, Xiaoning,Fu, Xin,&Yu, Changlong.(2011).Synergistic Inhibition of Endochondral Bone Formation by Silencing Hif1 alpha and Runx2 in Trauma-induced Heterotopic Ossification.MOLECULAR THERAPY,19(8),1426-1432.
MLA Lin, Lin,et al."Synergistic Inhibition of Endochondral Bone Formation by Silencing Hif1 alpha and Runx2 in Trauma-induced Heterotopic Ossification".MOLECULAR THERAPY 19.8(2011):1426-1432.
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