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学科主题基础医学
Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis
Rui, M; Chen, YY; Zhang, YM; Ma, DL
关键词TFAR19 in situ electroporation apoptosis antibody
刊名LIFE SCIENCES
2002-08-30
71期:15页:1771-1778
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]MAMMALIAN-CELLS ; TFAR19
英文摘要

Electroporation has been successfully used for the introduction of DNA, RNA, oligonucleotide and protein into eukaryotic and prokaryotic cells for the transformation and expression of various gene products. TFAR19 (TF-1 apoptosis-related gene 19), also designated PDCD5 (Programmed Cell Death 5), is cloned as an increased expression gene during the apoptotic process of TF-1 cell induced by cytokine withdrawal. It facilitates rather than induces apoptosis in different cell lines. To explore its molecular mechanism, we successfully transferred the anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation and observed the apoptosis process of HeLa cells induced by etoposide with flow cytometry. We demonstrate that the introduction of anti-TFAR19 antibody can suppress the apoptosis accelerating effect of TFAR19 in its natural environment. This study shows that TFAR19 may be a critical factor for apoptosis; and transfer of monoclonal antibody into mammalian cells by in situ electroporation is a useful method to study the function of endogeneous factors. (C) 2002 Elsevier Science Inc. All rights reserved.

语种英语
WOS记录号WOS:000177487200006
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被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58034
专题北京大学基础医学院_免疫学系
作者单位Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Rui, M,Chen, YY,Zhang, YM,et al. Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis[J]. LIFE SCIENCES,2002,71(15):1771-1778.
APA Rui, M,Chen, YY,Zhang, YM,&Ma, DL.(2002).Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis.LIFE SCIENCES,71(15),1771-1778.
MLA Rui, M,et al."Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis".LIFE SCIENCES 71.15(2002):1771-1778.
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