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学科主题: 基础医学
题名:
Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis
作者: Rui, M; Chen, YY; Zhang, YM; Ma, DL
关键词: TFAR19 ; in situ electroporation ; apoptosis ; antibody
刊名: LIFE SCIENCES
发表日期: 2002-08-30
卷: 71, 期:15, 页:1771-1778
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: MAMMALIAN-CELLS ; TFAR19
英文摘要:

Electroporation has been successfully used for the introduction of DNA, RNA, oligonucleotide and protein into eukaryotic and prokaryotic cells for the transformation and expression of various gene products. TFAR19 (TF-1 apoptosis-related gene 19), also designated PDCD5 (Programmed Cell Death 5), is cloned as an increased expression gene during the apoptotic process of TF-1 cell induced by cytokine withdrawal. It facilitates rather than induces apoptosis in different cell lines. To explore its molecular mechanism, we successfully transferred the anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation and observed the apoptosis process of HeLa cells induced by etoposide with flow cytometry. We demonstrate that the introduction of anti-TFAR19 antibody can suppress the apoptosis accelerating effect of TFAR19 in its natural environment. This study shows that TFAR19 may be a critical factor for apoptosis; and transfer of monoclonal antibody into mammalian cells by in situ electroporation is a useful method to study the function of endogeneous factors. (C) 2002 Elsevier Science Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000177487200006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58034
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Rui, M,Chen, YY,Zhang, YM,et al. Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis[J]. LIFE SCIENCES,2002,71(15):1771-1778.
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