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IR@PKUHSC  > 北京大学第三临床医学院  > 肿瘤放疗科  > 期刊论文
学科主题: 临床医学
题名:
The biological effect of I-125 seed continuous low dose rate irradiation in CL187 cells
作者: Zhuang, Hong-Qing1; Wang, Jun-Jie1; Liao, An-Yan1; Wang, Ji-Dong1; Zhao, Yong2
刊名: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
发表日期: 2009-01-29
DOI: 10.1186/1756-9966-28-12
卷: 28
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: GROWTH-FACTOR RECEPTOR ; RATE RADIATION ; INTERSTITIAL BRACHYTHERAPY ; PANCREATIC-CANCER ; TUMOR-CELLS ; IN-VITRO ; THERAPY ; CARCINOMA ; APOPTOSIS ; LINES
英文摘要:

Background: To investigate the effectiveness and mechanism of I-125 seed continuous low-dose-rate irradiation on colonic cell line CL187 in vitro.

Methods: The CL187 cell line was exposed to radiation of Co-60 gamma ray at high dose rate of 2 Gy/min and I-125 seed at low dose rate of 2.77 cGy/h. Radiation responses to different doses and dose rates were evaluated by colony-forming assay. Under I-125 seed low dose rate irradiation, a total of 12 culture dishes were randomly divided into 4 groups: Control group, and 2, 5, and 10 Gy irradiation groups. At 48 h after irradiation, apoptosis was detected by Annexin and Propidium iodide (PI) staining. Cell cycle arrests were detected by PI staining. In order to investigate the influence of low dose rate irradiation on the MAPK signal transduction, the expression changes of epidermal growth factor receptor (EGFR) and Raf under continuous low dose rate irradiation (CLDR) and/or EGFR monoclonal antibodies were determined by indirect immunofluorescence.

Results: The relative biological effect (RBE) for I-125 seeds compared with Co-60 gamma ray was 1.41. Apoptosis rates of CL187 cancer cells were 13.74% +/- 1.63%, 32.58% +/- 3.61%, and 46.27% +/- 3.82% after 2 Gy, 5 Gy, and 10 Gy irradiation, respectively; however, the control group apoptosis rate was 1.67% +/- 0.19%. G(2)/M cell cycle arrests of CL187 cancer cells were 42.59% +/- 3.21%, 59.84% +/- 4.96%, and 34.61% +/- 2.79% after 2 Gy, 5 Gy, and 10 Gy irradiation, respectively; however, the control group apoptosis rate was 26.44% +/- 2.53%. P < 0.05 vs. control groups by Student′s t-test were found in every treated group both in apoptosis and in G(2)/M cell cycle arrest. After low dose rate irradiation, EGFR and Raf expression increased, but when EGFR was blocked by a monoclonal antibody, EGFR and Raf expression did not change.

Conclusion: I-125 seeds resulted in more effective inhibition than Co-60 gamma ray high dose rate irradiation in CL187 cells. Apoptosis following G(2)/M cell cycle arrest was the main mechanism of cell-killing effects under low dose rate irradiation. CLDR could influence the proliferation of cells via MAPK signal transduction.

语种: 英语
项目资助者: Ministry of Civil Affair, China
WOS记录号: WOS:000264474500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58038
Appears in Collections:北京大学第三临床医学院_肿瘤放疗科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Radiat Oncol, Ctr Canc, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, Transplantat Biol Res Div, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100101, Peoples R China

Recommended Citation:
Zhuang, Hong-Qing,Wang, Jun-Jie,Liao, An-Yan,et al. The biological effect of I-125 seed continuous low dose rate irradiation in CL187 cells[J]. JOURNAL OF EXPERIMENTAL &amp; CLINICAL CANCER RESEARCH,2009,28.
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