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学科主题: 基础医学
题名:
Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis
作者: Huang, Jing; Zhang, Li; Ma, Teng; Zhang, Pei; Qiu, Xiaoyan
关键词: immunoglobulin ; V-(D)-J rearrangement ; mouse ; testis ; epididymis
刊名: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
发表日期: 2009-04-01
DOI: 10.1369/jhc.2008.951434
卷: 57, 期:4, 页:339-349
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: INDUCED CYTIDINE DEAMINASE ; ANTISPERM ANTIBODIES ; SPERM ; CELLS ; CHAIN ; IGG ; SPERMATOGENESIS ; CYTOKINES ; TRANSPORT ; PROTEINS
英文摘要:

Immunoglobulin (Ig) molecules have thus far been found only to be produced by differentiated B lymphocytes. As we know, immune privilege in the normal adult mouse testis exists to make these privileged sites generally free of patrolling immune cells and Igs. However, in this study, the expression of Ig in normal adult mouse testis and epididymis was detected. First, by IHC, a strong pattern of Ig expression was detected in the spermatogenic cells of adult mouse testis at different development stages. Second, by Western blot analysis, different strong bands of Igs in mouse testicular spermatogenic cells and epididymal epithelial cell extract were recognized using specific antibodies against IgG. More importantly, by ISH and cell sorting-related RT-PCR, rearranged Ig gamma chain and kappa chain transcripts were expressed in testicular spermatogenic cells and epididymal epithelial cells. These results suggested that Ig in testis and epididymis was mainly produced by adult mouse testicular spermatogenic cells and epididymal epithelial cells. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 57:339-349, 2009)

语种: 英语
所属项目编号: 30371609
项目资助者: National Natural Science Foundation, China
WOS记录号: WOS:000264257000005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58066
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

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作者单位: Peking Univ, Ctr Human Dis Genom, Inst Basic Med Sci, Beijing 100083, Peoples R China

Recommended Citation:
Huang, Jing,Zhang, Li,Ma, Teng,et al. Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY,2009,57(4):339-349.
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