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学科主题: 临床医学
题名:
Mammalian actin-binding protein 1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction
作者: He, Kangmin1,2,3,4; Xing, Rui1,2,3; Yan, Xiaohua5; Tian, Aiju1,2,3; Zhang, Mingliang1,2,3; Yuan, Jinghe4; Lv, Zhizhen1,2,3; Fang, Xiaohong4; Li, Zijian1,2,3; Zhang, Youyi1,2,3
关键词: membrane trafficking ; adaptor protein ; quantitative live-cell imaging
刊名: FASEB JOURNAL
发表日期: 2015-06-01
DOI: 10.1096/fj.14-264259
卷: 29, 期:6, 页:2495-2503
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
关键词[WOS]: MEDIATED ENDOCYTOSIS ; ADAPTER PROTEIN ; CYTOSKELETON ; ACTIVATION ; RECEPTORS ; GTPASE
英文摘要:

Actin and dynamin work cooperatively to drive the invagination and scission of clathrin-coated pits (CCPs). However, little is known about the mechanism that orchestrates the spatiotemporal recruitment of dynamin and actin. Here, we have identified the mammalian actin-binding protein 1 (mAbp1; also called HIP-55 or SH3P7), which could bind toclathrin, actin, as well as dynamin, as an adaptor that links the dynamic recruitment of dynamin and actin for the scission of CCPs. Live-cell imaging reveals that mAbp1 is specifically recruited at a late stage of the long-lived CCPs. mAbp1 knockdown impaired CCP scission by reducing dynamin recruitment at the plasma membrane. However, actin disruption remarkably eliminates mAbp1 recruitment and thus dynamin recruitment. These data suggest that by binding to both clathrin and F-actin, mAbp1 is specifically recruited at a late stage of CCP formation, which subsequently recruits dynamin to CCPs.

语种: 英语
所属项目编号: 2013CB933701 ; 2012CB518000 ; 2011CB503903 ; 81471893 ; 81070078 ; 81270157 ; 81270159
项目资助者: National Key Basic Research Program of the People&prime ; s Republic of China ; Natural Science Foundation of China ; Emory Global Health Institute (Atlanta, GA, USA)
WOS记录号: WOS:000355209500026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58086
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100871, Peoples R China
2.Peking Univ, Acad Adv Interdisciplinary Studies, Key Lab Cardiovascular Mol Biol & Regulatory Pept, Key Lab Mol Cardiovasc Sci,Minist Educ,Minist Hlt, Beijing 100871, Peoples R China
3.Beijing Key Lab Cardiovasc Receptors Res, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China
5.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China

Recommended Citation:
He, Kangmin,Xing, Rui,Yan, Xiaohua,et al. Mammalian actin-binding protein 1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction[J]. FASEB JOURNAL,2015,29(6):2495-2503.
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